A molecular survey of Anaplasma, Ehrlichia, Bartonella and Theileria in ticks collected from southeastern China
A molecular survey of Anaplasma, Ehrlichia, Bartonella and Theileria in ticks collected from southeastern China
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中国东南部蜱虫中无形体、埃利希体、巴尔通体和泰勒虫的分子调查
DOI：
10.1007/s10493-019-00411-2
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发表时间：
2019-09-01
影响因子：
2.2
通讯作者：
Gong, Zhenyu
中科院分区：
文献类型：
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作者：
Hou, Juan;Ling, Feng;Gong, Zhenyu
为了调查无形体、埃立克体、巴尔通体和泰勒虫的流行情况，我们从中国东南部浙江省的六个县的小型哺乳动物身上采集了蜱虫。对蜱虫样本进行聚合酶链反应（PCR）扩增以检测无形体、埃立克体、巴尔通体和泰勒虫。对阳性PCR产物进行测序，然后使用BLAST与先前在GenBank中公布的序列进行比对。共捕获了约292只成年蜱虫，优势蜱种为中华硬蜱和长角血蜱。总体而言，34只蜱虫（11.6%）至少对无形体、埃立克体、巴尔通体和泰勒虫中的一种病原体检测呈阳性。对无形体、埃立克体、巴尔通体和泰勒虫的PCR阳性率分别为5.5%、1.7%、2.4%和2.4%。不同种类蜱虫中无形体、巴尔通体和泰勒虫的阳性率存在显著差异。不同县的蜱虫中无形体和泰勒虫的流行率存在显著差异。无形体、埃立克体、巴尔通体和泰勒虫在浙江省的蜱虫中广泛流行，这表明如果患者有蜱虫叮咬史，也应怀疑其他蜱传病原体。
To investigate the prevalence of Anaplasma, Ehrlichia, Bartonella and Theileria, we collected ticks from small mammals in six counties of Zhejiang Province in southeastern China. Polymerase chain reaction (PCR) amplification was performed to test Anaplasma, Ehrlichia, Bartonella and Theileria in tick samples. Positive PCR products were sequenced and then compared with previously published sequences deposited in GenBank using BLAST. About 292 adult ticks were captured and the dominant tick species were Ixodes sinensis and Haemaphysalis longicornis. Overall, 34 ticks (11.6%) were tested positive for at least one pathogen of Anaplasma, Ehrlichia, Bartonella and Theileria. Rates of PCR-positivity to Anaplasma, Ehrlichia, Bartonella and Theileria were 5.5, 1.7, 2.4 and 2.4%, respectively. Positive rates of Anaplasma, Bartonella and Theileria were significantly different among ticks of different species. Prevalence of Anaplasma and Theileria varied significantly among ticks of different counties.