Fluorescent fiber optic calcium sensor for physiological measurements
Fluorescent fiber optic calcium sensor for physiological measurements
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DOI：
10.1021/ac950944k
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发表时间：
1996-04-15
影响因子：
7.4
通讯作者：
Hoyland, B
中科院分区：
文献类型：
--
作者：
Shortreed, M;Kopelman, R;Hoyland, B
一种新的光学传感器的基础上共价固定的新合成的钙选择性，长波长，荧光指示剂已建成，具有最佳的生理测量的响应动态范围。固定发生通过光引发共聚的指示剂与丙烯酰胺上的硅烷化的125 μ m直径的多模光纤的远端。该传感器的工作寿命仅受指示剂光漂白的限制。由于丙烯酰胺聚合物的固有亲水性，这种新传感器的响应时间由离子钙的简单水扩散控制，这导致传感器响应时间足够快以监测生理速率下的一些浓度波动。还证明了在高背景水平的镁中监测钙浓度波动的能力，计算的选择性为10（-4.5）。
A new optical sensor based on covalent immobilization of a newly synthesized calcium-selective, long-wavelength, fluorescent indicator has been constructed, with a response dynamic range optimal for physiological measurements, Immobilization occurs via photoinitiated copolymerization of the indicator with acrylamide on the distal end of a silanized 125 mu m diameter multimode optical fiber. The working lifetime of this sensor is limited only by photobleaching of the indicator. Due to the inherent hydrophilic nature of the acrylamide polymer, the response time of this new sensor is governed by simple aqueous diffusion of the ionic calcium, This results in sensor response times fast enough to monitor some concentration fluctuations at physiological rates. The ability to monitor calcium concentration fluctuations in a high background level of magnesium is also demonstrated with a calculated selectivity of 10(-4.5).