Investigation of the induction of antibodies against Crandell-Rees feline kidney cell lysates and feline renal cell lysates after parenteral administration of vaccines against feline viral rhinotracheitis, calicivirus, and panleukopenia in cats
Investigation of the induction of antibodies against Crandell-Rees feline kidney cell lysates and feline renal cell lysates after parenteral administration of vaccines against feline viral rhinotracheitis, calicivirus, and panleukopenia in cats
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DOI：
10.2460/ajvr.2005.66.506
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发表时间：
2005-03-01
影响因子：
1
通讯作者：
Hawley, JR
中科院分区：
文献类型：
--
作者：
Lappin, MR;Jensen, WA;Hawley, JR
目的：研究Crandell-Rees猫肾(CRFK)细胞裂解物或可能含有CRFK细胞蛋白的猫病毒性鼻气管炎、杯状病毒和泛白细胞减少症疫苗(FVRCP疫苗)是否能诱导猫、动物-14只8周龄猫的抗CRFK细胞或猫肾细胞(FRC)裂解物抗体。分别在0、3、6和50周给2只猫接种4种FVRCP疫苗。在0到50周期间，另外3对猫接受了11种CRFK细胞裂解液SC(10、50或50杯与明矾混合)接种。每隔一段时间进行临床病理评估和血清抗CRFK细胞或FRC裂解物抗体的ELISA检测。所有注射CRFK细胞裂解物的猫都多次检测到针对CRFK细胞或FRC裂解物的抗体。在6只非肠道接种的猫中，5只至少有一次可检测到抗CRFK细胞裂解物的抗体，但所有6只猫都有多次可检测到的抗FRC裂解物抗体。接受鼻腔-眼内疫苗注射的猫没有产生可检测到的针对这两种裂解物的抗体。在研究期间没有发现重要的临床病理或组织学异常。结论和临床相关性-注射含有可能生长在CRFK细胞上的病毒的疫苗在猫中诱导针对CRFK细胞和FRC裂解物的抗体。在这项为期56周的研究中，CRFK细胞蛋白的超敏反应没有导致猫的肾脏疾病。
Objective-To determine whether administration of Crandell-Rees feline kidney (CRFK) cell lysates or vaccines against feline viral rhinotracheitis, calicivirus, and panleukopenia (FVRCP vaccines) that likely contain CRFK cell proteins induces antibodies against CRFK cell or feline renal cell (FRC) lysates in cats,Animals-14 eight-week-old cats.Procedure-Before and after the study, renal biopsy specimens were obtained from each cat for histologic evaluation. Each of 4 FVRCP vaccines was administered to 2 cats at weeks 0, 3, 6, and 50. Between weeks 0 and 50, another 3 pairs of cats received 11 CRFK cell lysate inoculations SC (10, 50, or 50 mug mixed with alum). Clinicopathologic evaluations and ELISAs to detect serum antibodies against CRFK cell or FRC lysates were performed at intervals.Results-Cats had no antibodies against CRFK cell or FRC lysates initially. All cats administered CRFK cell lysate had detectable antibodies against CRFK cell or FRC lysates on multiple occasions. Of 6 cats vaccinated parenterally, 5 had detectable antibodies against CRFK cell lysate at least once, but all 6 had detectable antibodies against FRC lysate on multiple occasions. Cats administered an intranasal-intraocular vaccine did not develop detectable antibodies against either lysate. Important clinicopathologic or histologic abnormalities were not detected during the study.Conclusions and Clinical Relevance-Parenteral administration of vaccines containing viruses likely grown on CRFK cells induced antibodies against CRFK cell and FRC lysates in cats. Hypersensitization with CRFK cell proteins did not result in renal disease in cats during the 56-week study.