A comparison of cell killing by heat and/or X rays in Chinese hamster V79 cells, Friend erythroleukemia mouse cells, and human thymocyte MOLT-4 cells.
A comparison of cell killing by heat and/or X rays in Chinese hamster V79 cells, Friend erythroleukemia mouse cells, and human thymocyte MOLT-4 cells.
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中国仓鼠 V79 细胞、Friend 红白血病小鼠细胞和人胸腺细胞 MOLT-4 细胞中热和/或 X 射线细胞杀伤的比较。
DOI：
10.2307/3575968
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发表时间：
1983
影响因子：
3.4
通讯作者：
E. Azzam
中科院分区：
文献类型：
--
作者：
G. Raaphorst;J. Székely;A. Lobreau;E. Azzam
比较了中国仓鼠 V79 细胞、Friend 红白血病 (FELC) 小鼠细胞和 MOLT-4 人转化胸腺细胞的辐射和/或热敏感性。 MOLT-4 细胞比 FELC (D0 = 0.65 Gy) 和 V79 细胞 (D0 = 1.43 Gy) 更具放射敏感性 (D0 = 0.50 Gy)。 Arrhenius分析表明，MOLT-4细胞在42.0℃以下比FELC或V79细胞更热敏感，但在更高温度下更耐热。此外，MOLT-4细胞在41.0至45.0℃之间显示出单次热失活能，而FELC和V79细胞分别在约43.0和43.5℃处显示出失活能转变。这些差异可能与以下事实有关：MOLT-4 电池在连续加热过程中产生热耐受性的温度上限低于 FELC 或 V79 电池。 FELC 和 V79 细胞的杀灭取决于热和 X 射线的施加顺序，但同时给予两种处理时可获得最大效果。当通过在 37.0 摄氏度下孵育分开处理时，出现恢复。MOLT-4 细胞没有表现出加热和照射的序列依赖性。使用台盼蓝染料排斥或集落形成来比较加热和/或照射后MOLT-4细胞的存活率。两种测定均显示出相似的定性反应，但台盼蓝测定测定的存活水平远高于集落形成测定测定的存活水平。
The radiation and/or heat sensitivity of Chinese hamster V79 cells, Friend erythroleukemia (FELC) mouse cells, and MOLT-4 human transformed thymocytes were compared. MOLT-4 cells were more radiosensitive (D0 = 0.50 Gy) than FELC (D0 = 0.65 Gy) and V79 cells (D0 = 1.43 Gy). Arrhenius analysis showed that MOLT-4 cells were more heat sensitive than FELC or V79 cells below 42.0 degrees C, but more heat resistant at higher temperatures. In addition, the MOLT-4 cells showed a single-heat inactivation energy between 41.0 and 45.0 degrees C, while FELC and V79 cells both showed a transition in the inactivation energy at about 43.0 and 43.5 degrees C, respectively. These differences may be related to the fact that the upper temperature limit for the development of thermal tolerance during continuous heating was lower for MOLT-4 cells than for FELC or V79 cells. Killing of FELC and V79 cells was dependent on the sequence in which heat and X rays were applied, but the greatest effect was obtained when both treatments were given simultaneously. Recovery occurred when treatments were separated by incubation at 37.0 degrees C. The MOLT-4 cells did not show a sequence dependence for heating and irradiation. Survival of MOLT-4 cells after heating and/or irradiation was compared using trypan blue dye exclusion or colony formation. Both assays showed similar qualitative responses, but survival levels measured by the trypan blue assay were much higher than those determined from the colony-forming assay.