Proteomic identification of potential protein markers in cerebrospinal fluid of GBS patients
Proteomic identification of potential protein markers in cerebrospinal fluid of GBS patients
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DOI：
10.1111/j.1468-1331.2007.01761.x
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发表时间：
2007-05-01
影响因子：
5.1
通讯作者：
Zhu, J.
中科院分区：
文献类型：
--
作者：
Jin, T.;Hu, L. -S.;Zhu, J.
脑脊液（CSF）中蛋白质水平升高是格林-巴利综合征（GBS）患者的特征，GBS是一种外周神经系统（PNS）的急性炎症性自身免疫性疾病。然而，该疾病的分子机制仍然知之甚少，到目前为止还没有可靠的疾病相关标志物。通过比较GBS患者与患有其他神经系统疾病的对照受试者的CSF蛋白质组，有可能鉴定参与疾病过程的蛋白质，从而研究GBS的发病机制。我们采用二维差异凝胶电泳（2DIGE）技术，结合基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）技术，对GBS患者脑脊液中的异常蛋白进行了检测。我们的数据显示，GBS患者与对照组相比，CSF中六种蛋白质及其亚型的水平显著改变。GBS患者CSF中结合珠蛋白、载脂蛋白A-IV和PRO 2044（未命名蛋白）显著升高，而甲状腺素运载蛋白、载脂蛋白E和纤维蛋白原显著降低。我们的结论是，这六个蛋白可能参与了GBS的发病机制，并呼吁进一步研究这些蛋白在疾病的发病机制中的作用。
Increased protein level in the cerebrospinal fluid (CSF) is a characteristic of patients with Guillain-Barre syndrome (GBS), an acute inflammatory autoimmune disorder in the peripheral nervous system (PNS). However, the molecular mechanisms underlying the disease remain poorly understood and so far no reliable disease-related markers are available. By comparing the CSF proteome of GBS patients with control subjects suffering from other neurological disorders, it may be possible to identify proteins that involve in the disease process and thus to study the pathogenesis of GBS. We used two-dimensional difference gel electrophoresis (2D DIGE) technique, in combination with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS), to determine the abnormal CSF proteins in GBS patients. Our data showed that the levels of six proteins and their isoforms in CSF were significantly altered in GBS patients compared with controls. Haptoglobin, apolipoprotein A-IV and PRO2044 (unnamed protein) were considerably increased in the CSF of GBS patients, whereas transthyretin, apolipoprotein E and fibrinogen were considerably decreased. We concluded that these six proteins may be involved in the pathogenesis of GBS and call for further studying the role of these proteins in the pathogenesis of the disease.