Advanced bioreactor with controlled application of multi-dimensional strain for tissue engineering
Advanced bioreactor with controlled application of multi-dimensional strain for tissue engineering
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DOI：
10.1115/1.1519280
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发表时间：
2002-12-01
影响因子：
1.7
通讯作者：
Vunjak-Novakovic, G
中科院分区：
文献类型：
--
作者：
Altman, GH;Lu, HH;Vunjak-Novakovic, G
先进的生物反应器对于满足体外工程功能性骨骼组织的复杂要求是必不可少的。为了满足这一需求，我们开发了一种计算机控制的台式生物反应器系统，该系统能够将复杂的并发机械应变应用于独立容纳在24个反应器容器中的三维基质，并结合增强的环境和流体控制。我们证明了这种新系统的潜力，以解决组织工程的需求，特别是对发展的组织工程前交叉韧带从人骨髓基质细胞（hBMSC），其中复杂的机械和生化环境控制是必不可少的组织功能。良好控制的机械应变（
Advanced bioreactors are essential for meeting the complex requirements of in vitro engineering functional skeletal tissues. To address this need, we have developed a computer controlled bench-top bioreactor system with capability to apply complex concurrent mechanical strains to three-dimensional matrices independently housed in 24 reactor vessels, in conjunction with enhanced environmental and fluidic control. We demonstrate the potential of this new system to address needs in tissue engineering, specifically toward the development of a tissue engineered anterior cruciate ligament from human bone-marrow stromal cells (hBMSC), where complex mechanical and biochemical environment control is essential to tissue function. Well-controlled mechanical strains (resolution of