Octopamine enhances oxidative stress resistance through the fasting-responsive transcription factor DAF-16/FOXO in C. elegans
Octopamine enhances oxidative stress resistance through the fasting-responsive transcription factor DAF-16/FOXO in C. elegans
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DOI：
10.1111/gtc.12469
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发表时间：
2017-02-01
期刊：
影响因子：
2.1
通讯作者：
Nishida, Eisuke
中科院分区：
文献类型：
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作者：
Hoshikawa, Haruka;Uno, Masaharu;Nishida, Eisuke
饮食限制方案通过调节禁食和/或饮食反应机制，增强了许多物种的抗应激能力，延长了寿命。禁食刺激被感觉神经元感知，并引起行为和代谢适应。章鱼胺（OA）是秀丽隐杆线虫的神经递质之一，参与行为适应，其水平在禁食条件下增加。然而，OA如何促进空腹反应在很大程度上仍然未知。在这项研究中，我们发现，OA管理增强机体抗氧化应激。这种增强的抵抗力被OA受体SER-3和SER-6的突变所抑制。此外，我们发现OA给药促进了禁食反应中的关键转录因子β-16的核转位，并且OA诱导的应激抗性增强需要β-16。总而言之，我们的结果表明，由食物缺乏引发的OA信号将生物体状态转变为更具保护性的状态，以应对环境压力。
Dietary restriction regimens lead to enhanced stress resistance and extended life span in many species through the regulation of fasting and/or diet-responsive mechanisms. The fasting stimulus is perceived by sensory neurons and causes behavioral and metabolic adaptations. Octopamine (OA), one of the Caenorhabditis elegans neurotransmitters, is involved in behavioral adaptations, and its levels are increased under fasting conditions. However, it remains largely unknown how OA contributes to the fasting responses. In this study, we found that OA administration enhanced organismal resistance to oxidative stress. This enhanced resistance was suppressed by a mutation of the OA receptors, SER-3 and SER-6. Moreover, we found that OA administration promoted the nuclear translocation of DAF-16, the key transcription factor in fasting responses, and that the OA-induced enhancement of stress resistance required DAF-16. Altogether, our results suggest that OA signaling, which is triggered by the absence of food, shifts the organismal state to a more protective one to prepare for environmental stresses.