Protective effect of HLA-B57 on HCV genotype 2 infection in a West African population
Protective effect of HLA-B57 on HCV genotype 2 infection in a West African population
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DOI：
10.1002/jmv.20848
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发表时间：
2007-06-01
影响因子：
12.7
通讯作者：
Allain, Jean-Pierre
中科院分区：
文献类型：
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作者：
Chuang, Wing Chia-Min;Sarkodie, Francis;Allain, Jean-Pierre
丙型肝炎病毒（HCV）感染的恢复在西方人群中被认为是罕见的（< 20%），但在西非达到50%，其中基因型2感染占主导地位。为了研究细胞免疫反应和宿主遗传学在这一现象中的作用，在2000年至2005年期间收集了104名加纳献血者的抗HCV检测反应样本。HCV抗体通过使用基因型2重组核心、E2和NS 3蛋白的Western印迹来确认。测定病毒载量和基因型。样本分为37个慢性，35个恢复感染和32个假阳性。81%的慢性感染（RNA阳性）受试者携带基因型2 HCV。对35例适合于干扰素-γ ELISPOT检测的冷冻外周血单个核细胞（PBMC）进行了细胞免疫反应研究。24例中有12例确认恢复，5例中有1例慢性感染，6例假阳性对照均未对重组蛋白产生反应。DNA分型法检测HLA-A、-B、-DR型。HCV感染恢复组HLA-13*57阳性率明显高于慢性感染组（P= 0.0053，OR = 8.02）。总之，据推测，基因型2型HCV毒株的优势可能是一个重要因素，解释了高回收率的HCV感染在加纳通过一个有效的贡献HLA-13*57，这是相对频繁的人口。医学病毒学杂志。79;724- 733，2007。(C)2007 Wiley-Liss，Inc.
Recovery from Hepatitis C virus (HCV) infection is considered infrequent (< 20%) in western populations but reaches 50% in West Africa where genotype 2 infection is predominant. To investigate the role of cellular immune responses and host genetics in this phenomenon, samples from 104 Ghanaian blood donors reactive with anti-HCV assays were collected between 2000 and 2005. HCV antibody was confirmed by Western blot using genotype 2 recombinant core, E2 and NS3 proteins. Viral load and genotype were determined. Samples were stratified into 37 chronic, 35 recovered infections and 32 false positive. Eighty-one percentage of subjects with chronic infection (RNA positive) carried genotype 2 HCV. Cellular immune response was investigated in 35 frozen peripheral blood mononuclear cell (PBMC) samples suitable for interferon-gamma ELISPOT assay. Twelve out of 24 confirmed recovered, 1 out of 5 chronically infected and none of the 6 false-positive controls reacted to recombinant proteins. HLA-A, -B and -DR types were determined by DNA methodology. HLA-13*57 was significantly more frequent in the group which had recovered from HCV infection compared with chronically infected subjects (P= 0.0053, OR = 8.02). In conclusion, it is hypothesized that the dominance of genotype 2 HCV strains may be an important factor explaining the high rate of recovery from HCV infections in Ghana via an efficient contribution of HLA-13*57 which is relatively frequent in the population. J. Med Virol. 79;724-733,2007. (C) 2007 Wiley-Liss, Inc.