Osteogenic differentiation of human mesenchymal stem cells promotes mineralization within a biodegradable peptide hydrogel.
Osteogenic differentiation of human mesenchymal stem cells promotes mineralization within a biodegradable peptide hydrogel.
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DOI：
10.1177/2041731416649789
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发表时间：
2016-01
影响因子：
8.2
通讯作者：
Miller AF
中科院分区：
文献类型：
--
作者：
Castillo Diaz LA;Elsawy M;Saiani A;Gough JE;Miller AF
一种有吸引力的组织再生策略是使用细胞外基质类似的生物材料。基于肽的纤维状水凝胶已经显示出模拟细胞外基质的结构，为细胞提供了承担其生理功能的小生境。在这项研究中，离子互补肽FEFEFKFK（F，E和K分别是苯丙氨酸，谷氨酸和赖氨酸）水凝胶在三维空间中宿主人间充质干细胞并诱导其成骨分化的能力得到证明。分析显示，在12天的培养中，整个水凝胶中的细胞活力和增殖持续存在，并且这些人间充质干细胞在加入成骨刺激后简单地分化成成骨细胞。分化的成骨细胞合成关键的骨蛋白，包括胶原蛋白-1（Col-1）、骨钙素和碱性磷酸酶。此外，矿化发生在水凝胶内。肽水凝胶是一种天然可生物降解的材料，如振荡流变学和反相高效液相色谱法所示，其中分别随时间监测水凝胶的粘弹性和降解。这些发现表明，生物可降解的八肽水凝胶可以宿主和诱导干细胞分化，并具有硬组织如牙槽骨再生的潜力。
An attractive strategy for the regeneration of tissues has been the use of extracellular matrix analogous biomaterials. Peptide-based fibrillar hydrogels have been shown to mimic the structure of extracellular matrix offering cells a niche to undertake their physiological functions. In this study, the capability of an ionic-complementary peptide FEFEFKFK (F, E, and K are phenylalanine, glutamic acid, and lysine, respectively) hydrogel to host human mesenchymal stem cells in three dimensions and induce their osteogenic differentiation is demonstrated. Assays showed sustained cell viability and proliferation throughout the hydrogel over 12 days of culture and these human mesenchymal stem cells differentiated into osteoblasts simply upon addition of osteogenic stimulation. Differentiated osteoblasts synthesized key bone proteins, including collagen-1 (Col-1), osteocalcin, and alkaline phosphatase. Moreover, mineralization occurred within the hydrogel. The peptide hydrogel is a naturally biodegradable material as shown by oscillatory rheology and reversed-phase high-performance liquid chromatography, where both viscoelastic properties and the degradation of the hydrogel were monitored over time, respectively. These findings demonstrate that a biodegradable octapeptide hydrogel can host and induce the differentiation of stem cells and has the potential for the regeneration of hard tissues such as alveolar bone.