Changes in cell dimensions and intercellular contacts during cleavage-stage cell cycles in mouse embryonic cells.
Changes in cell dimensions and intercellular contacts during cleavage-stage cell cycles in mouse embryonic cells.
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小鼠胚胎细胞分裂期细胞周期期间细胞尺寸和细胞间接触的变化。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
By E. Lehtonen
中科院分区：
文献类型：
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作者：
By E. Lehtonen
使用延时视频设备研究了从 1 至 8 细胞阶段小鼠胚胎中分离的细胞的裂解行为；记录细胞尺寸的变化及其时间。孤立细胞的分裂导致双细胞对的形成。这两个细胞的分裂总是异步的。在每次分裂中，子细胞的体积大约是亲本细胞的一半，但其表观表面积是亲本细胞的59-65%。因此，每个分区的表观表面积与体积之比增加了 25-30%。最明显的变化是在每次分裂的两个子细胞之间的关系中观察到的。胞质分裂后，2/8-和2/16-细胞对中的细胞间接触面积在接下来的细胞周期中逐渐增加，而2/2-和2/4-细胞对中几乎没有变化。子细胞在不同基质上行为的比较表明透明带和中体可能在早期接触发育中发挥作用。扫描电子显微镜用于研究细胞表面微绒毛密度的变化，试图解释细胞如何调节细胞间接触。
The cleavage behavior of cells isolated from 1- to 8-cell-stage mouse embryos was studied with time-lapse video equipment; changes in cellular dimensions and their timing were recorded. The division of an isolated cell results in the formation of a twin-cell pair. The divisions of these two cells were always asynchronous. In each division the volume of a daughter cell was approximately half of that of the parental cell but its apparent surface area was 59-65% of that of the parental cell. Consequently, the ratio of apparent surface area to volume increased in each division by 25-30%. The most noticeable changes were observed in the relationship between the two daughter cells of each division. After cytokinesis, the intercellular contact area gradually increased during the following cell cycle in the 2/8- and 2/16-cell pairs, whereas it hardly changed in the 2/2- and 2/4-cell pairs. The comparison of the behaviour of the daughter cells on different substrates suggested that the zona pellucida and the mid body might have a role in the contact development at the early stages. Scanning electron microscopy was used for studying changes in the density of cell surface microvilli in an attempt to explain how the cells regulate their intercellular contacts.