Antimicrobial Susceptibility Profiles of Human Campylobacter jejuni Isolates and Association with Phylogenetic Lineages.
Antimicrobial Susceptibility Profiles of Human Campylobacter jejuni Isolates and Association with Phylogenetic Lineages.
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DOI：
10.3389/fmicb.2016.00589
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发表时间：
2016
影响因子：
5.2
通讯作者：
Manning SD
中科院分区：
文献类型：
--
作者：
Cha W;Mosci R;Wengert SL;Singh P;Newton DW;Salimnia H;Lephart P;Khalife W;Mansfield LS;Rudrik JT;Manning SD
空肠弯曲菌是一种人畜共患病原体，也是全世界人类胃肠炎最常见的细菌原因。随着首选治疗药物氟喹诺酮类和大环内酯类抗生素耐药性的增加，空肠弯曲菌最近被列为严重的抗菌药物耐药威胁。在这里，我们对 2011 年和 2012 年从密歇根四家医院的患者收集的 94 个空肠弯曲菌分离株进行了特征分析，以确定耐药频率以及与系统发育谱系的关联。密歇根州空肠弯曲杆菌分离株中对氟喹诺酮类药物 (19.1%) 和大环内酯类药物 (2.1%) 的耐药率与国家报告相似。然而，从有国外旅行史的患者体内发现了高频率的耐氟喹诺酮类空肠弯曲菌。这些耐药菌株中很大一部分被归类为多位点序列类型 (ST)-464，这是最近在欧洲出现的一种氟喹诺酮耐药谱系。密歇根州还发现耐四环素空肠弯曲菌的患病率显着较高，并且耐药分离株更有可能代表 ST-982，ST-982 之前已从美国的反刍动物和环境中回收。值得注意的是，耐四环素空肠弯曲菌感染的患者更有可能与牛接触。这些结果提示需要监测进口的耐氟喹诺酮类空肠弯曲菌菌株的传播和多样化，并研究从牛和农场环境中回收的空肠弯曲菌的分子流行病学，以指导缓解策略。
Campylobacter jejuni is a zoonotic pathogen and the most common bacterial cause of human gastroenteritis worldwide. With the increase of antibiotic resistance to fluoroquinolones and macrolides, the drugs of choice for treatment, C. jejuni was recently classified as a serious antimicrobial resistant threat. Here, we characterized 94 C. jejuni isolates collected from patients at four Michigan hospitals in 2011 and 2012 to determine the frequency of resistance and association with phylogenetic lineages. The prevalence of resistance to fluoroquinolones (19.1%) and macrolides (2.1%) in this subset of C. jejuni isolates from Michigan was similar to national reports. High frequencies of fluoroquinolone-resistant C. jejuni isolates, however, were recovered from patients with a history of foreign travel. A high proportion of these resistant isolates were classified as multilocus sequence type (ST)-464, a fluoroquinolone-resistant lineage that recently emerged in Europe. A significantly higher prevalence of tetracycline-resistant C. jejuni was also found in Michigan and resistant isolates were more likely to represent ST-982, which has been previously recovered from ruminants and the environment in the U.S. Notably, patients with tetracycline-resistant C. jejuni infections were more likely to have contact with cattle. These outcomes prompt the need to monitor the dissemination and diversification of imported fluoroquinolone-resistant C. jejuni strains and to investigate the molecular epidemiology of C. jejuni recovered from cattle and farm environments to guide mitigation strategies.