Stereology of nerve cross sections
Stereology of nerve cross sections
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DOI：
10.1016/s0165-0270(98)00129-0
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发表时间：
1998-11-01
影响因子：
3
通讯作者：
Larsen, JO
中科院分区：
文献类型：
--
作者：
Larsen, JO
神经横截面的定量形态学数据在实验、发育和病理学研究中具有重要价值。有效的无偏体视学技术来估计从一个小样本的轴突的有髓和无髓轴突的总数和绝对大小分布的描述和评价。轴突数量估计与分馏技术。轴突面积估计与2D-nucleator和点计数技术的有髓和无髓轴突，分别。轴突周长估计使用的线网格，和髓鞘厚度的直接正交测量均匀，随机位置。当计数约200个轴突时，有髓鞘轴突的数量估计的经验评估方差类似于0.05。用四向2D成核剂获得的面积估计的经验方差与0.10相似，并且对动物内观察到的轴突面积的总方差的贡献小于0.5%。一个光学显微镜修改体视学与计算机辅助体视学测试系统和一个机动的阶段，促进有髓鞘轴突，这是在大多数研究的主要兴趣的数据采集。在更耗时的电子显微镜水平上评估无髓鞘轴突。(C)1998 Elsevier Science B. V.保留所有权利。
Quantitative morphological data from nerve cross sections are of great value in experimental, developmental, and pathological studies. Efficient unbiased stereological techniques to estimate the total numbers and absolute size distributions of both myelinated and unmyelinated axons from a small sample of axons are described and evaluated. Axon numbers are estimated with the fractionator technique. Axon areas are estimated with the 2D-nucleator and the point counting technique for myelinated and unmyelinated axons, respectively. The axon perimeter is estimated by use of a line grid, and the myelin sheath thickness by direct orthogonal measurements in uniform, random locations. The empirically evaluated variance of the number estimates of myelinated axons was similar to 0.05 when approximately 200 axons were counted. The empirical Variance of area estimates obtained with a four-way 2D-nucleator was similar to 0.10 and contributed less than 0.5% to the observed total variance of axon areas within an animal. A light microscope modified for stereology with computer assisted stereological test systems and a motorised stage facilitate the acquisition of data for the myelinated axons, which is of primary interest in most studies. The unmyelinated axons are evaluated at the more time consuming electron microscopical level. (C) 1998 Elsevier Science B.V. All rights reserved.