Potassium and sodium uptake systems in fungi. The transporter diversity of Magnaporthe oryzae
Potassium and sodium uptake systems in fungi. The transporter diversity of Magnaporthe oryzae
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DOI：
10.1016/j.fgb.2011.03.002
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发表时间：
2011-08-01
影响因子：
3
通讯作者：
Rodriguez-Navarro, Alonso
中科院分区：
文献类型：
--
作者：
Benito, Begona;Garciadeblas, Blanca;Rodriguez-Navarro, Alonso
在这项研究中，我们报告了62个真菌物种的K+吸收系统的清单，这些物种的完整基因组序列是可用的。这份清单揭示了三种类型的K+吸收系统，TRK和HAK转运体和ACU ATPase，广泛存在于真菌物种的几种组合中。PAT-ATPase的出现频率较低，在子囊菌中较为罕见。稻瘟病菌基因组含有4个TRK基因、1个Hak基因和2个ACU基因。对这些基因在高K+、K+饥饿和感染水稻叶片中表达的研究表明，除K饥饿外，ACU1、ACU2、HAK1和TRK1四个基因的表达明显低于TRK2、TRK3和TRK4。克隆了这两个ACU ATPase，并从功能上鉴定为高亲和力的K+或Na+摄取系统。这两种ATPase赋予酿酒酵母在缺K+时，在低Na+浓度下生长数代的能力，从而使细胞内Na+/K+比值显著增加。(C)2011 Elsevier Inc.保留所有权利。
In this study, we report an inventory of the K+ uptake systems in 62 fungal species for which the complete genome sequences are available. This inventory reveals that three types of K+ uptake systems, TRK and HAK transporters and ACU ATPases, are widely present in several combinations across fungal species. PAT ATPases are less frequently present and are exceptional in Ascomycota. The genome of Magnaporthe oryzae contains four TRK, one HAK, and two ACU genes. The study of the expression of these genes at high K+, K+ starvation, and in infected rice leaves revealed that the expression of four genes, ACU1, ACU2, HAK1, and TRK1 is much lower than that of TRK2, TRK3, and TRK4, except under K. starvation. The two ACU ATPases were cloned and functionally identified as high-affinity K+ or Na+ uptake systems. These two ATPases endow Saccharomyces cerevisiae with the capacity to grow for several generations in low Na+ concentrations when K+ was absent, which produces a dramatic increase of cellular Na+/K+ ratio. (C) 2011 Elsevier Inc. All rights reserved.