Expression and Syntenic Analyses of Four nanos Genes in Medaka
Expression and Syntenic Analyses of Four nanos Genes in Medaka
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DOI：
10.2108/zsj.26.112
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发表时间：
2009-02-01
影响因子：
0.9
通讯作者：
Tanaka, Minoru
中科院分区：
文献类型：
--
作者：
Aoki, Yumiko;Nakamura, Shuhei;Tanaka, Minoru
纳米基因对生殖细胞的发育至关重要。虽然纳米基因的功能和表达已经在小鼠中进行了研究，但在其他脊椎动物中尚未得到很好的表征。基于相似性和纳米序列的合成分析，我们在稻科植物（Oryzias latipes）基因组中鉴定出4个不同的纳米序列。Nanos1在硬骨鱼基因组中被复制，命名为nanos1a和nanos1b。在所有的medaka nanos中，nanos3在表达和合成方面具有较好的保守性。与先前对小鼠的研究相反，在性别分化的早期阶段，在性腺中未检测到nanos2的表达；然而，成年性腺的卵原细胞和精原细胞均表达nanos2。Nanos1a和1b都在发育中的大脑中表达，与小鼠中nanos1的表达一致。在性腺中，nanos1a在卵母细胞和精母细胞周围的体细胞中表达，而原位杂交在性腺中未检测到nanos1b的表达。这些结果表明纳米基因在脊椎动物中具有共同和独特的功能。
The gene nanos is essential for germ cell development. Although its functions and expression have been investigated in the mouse, nanos genes have yet to be well characterized in other vertebrates. Based on similarity and a syntenic analysis of nanos, we have identified four different nanos in the genome of medaka (Oryzias latipes). Nanos1 is duplicated in teleost fish genomes and named nanos1a and nanos1b. Of all medaka nanos, nanos3 is well conserved in terms of expression and synteny. In contrast to a previous study on mice, nanos2 expression was not detected in the gonads at early stages of sex differentiation; however, both oogonia and spermatogonia in adult gonads exhibit nanos2 expression. Nanos1a and 1b are both expressed in the developing brain, consistent with the expression of nanos1 in mice. In the gonads, nanos1a is expressed in the somatic cells surrounding oocytes and spermatocytes, whereas expression of nanos1b is not detectable in the gonads by in-situ hybridization. These results suggest common and distinct functions of nanos genes among vertebrates.