Determination of microvascular oxyhemoglobin saturations using cryospectrophotometry.
Determination of microvascular oxyhemoglobin saturations using cryospectrophotometry.
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使用冷冻分光光度法测定微血管氧合血红蛋白饱和度。
DOI：
10.1152/ajpheart.1990.259.6.h1912
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Gayeski,TE
中科院分区：
文献类型：
--
作者：
Fenton,BM;Gayeski,TE
尽管以前已经描述了一种用于测量血管内氧合血红蛋白(HBO2)饱和度的四波长低温分光光度方法，但实验测量与理论之间的关系还没有清楚地详细说明。目前的工作利用了HBO2饱和度测量和反射光血氧仪之间的经验关系，这与Kubelka和Munk(Z.Tech.太棒了。11A：593-603,1931年)。为了得到线性的、与浓度无关的校准曲线，理论结果要求1)与光密度、散射和吸收有关的复函数可以在所用的散射系数和吸收系数范围内线性逼近；以及2)散射系数与波长无关。入射光不容易用反射光谱测量，这排除了确定等色点的可能性。因此，使用了光密度差随饱和度不变的等波长对。这允许在540-600 nm范围内选择多个波长集合，而不是有限的等色散波长选择。最后讨论了冻结速度、冻结深度、Hb浓度和容器直径对实验测量的影响。
Although a four-wavelength method for cryospectrophotometric measurement of intravascular oxyhemoglobin (HbO2) saturations has previously been described, the relationship between experimental measurements and theory has not been clearly detailed. The current work utilizes an empirical relationship between HbO2 saturation measurements and reflected light oximetry, which is consistent with the two-flux theory of Kubelka and Munk (Z. Tech. Phys. 11a: 593-603, 1931). To obtain linear, concentration-independent calibration curves, the theoretical results require that 1) the complex function relating optical density, scattering, and absorption can be linearly approximated over the range of scattering and absorption coefficients used; and 2) the scattering coefficient is independent of wavelength. Incident light cannot easily be measured using reflection spectroscopy, which precludes the determination of isosbestic points. Therefore, equibestic wave-length pairs were used at which optical density differences were invariant with saturation. This allows numerous wavelength sets over the range 540-600 nm to be selected, rather than the limited choices of isosbestic wavelengths. Finally, the effects of freeze rate, freeze depth, Hb concentration, and vessel diameter are each discussed in terms of their influence on experimental measurements.