TAURINE AND HYPOTAURINE: THEIR EFFECTS ON MOTILITY, CAPACITATION AND THE ACROSOME REACTION OF HAMSTER SPERM IN VITRO AND THEIR PRESENCE IN SPERM AND REPRODUCTIVE TRACT FLUIDS OF SEVERAL MAMMALS *
TAURINE AND HYPOTAURINE: THEIR EFFECTS ON MOTILITY, CAPACITATION AND THE ACROSOME REACTION OF HAMSTER SPERM IN VITRO AND THEIR PRESENCE IN SPERM AND REPRODUCTIVE TRACT FLUIDS OF SEVERAL MAMMALS *
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牛磺酸和亚牛磺酸：它们对仓鼠精子体外活力、获能和顶体反应的影响以及它们在几种哺乳动物精子和生殖道液体中的存在*
DOI：
10.1111/j.1440-169x.1980.00483.x
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发表时间：
1980
期刊：
影响因子：
4.6
通讯作者：
R. Mrsny
中科院分区：
文献类型：
--
作者：
S. Meizel;C. W. Lui;P. Working;R. Mrsny
该实验室先前的工作表明，在存在或不存在肾上腺素的情况下，β-氨基酸牛磺酸可以在体外获能期间支持和刺激仓鼠精子活力。本报告描述了体外结果，表明亚牛磺酸，牛磺酸的前体，也可以支持和刺激运动在这些条件下，并发生更多的顶体反应，在牛磺酸的存在下相比，亚牛磺酸（在存在和不存在肾上腺素）。在所有的情况下，最大比例的顶体反应发生在肾上腺素的存在下。这些β-氨基酸是否独立于肾上腺素发挥作用，或与肾上腺素协同作用，仍有待确定。除了这些体外研究外，我们还报告了牛卵泡液、兔子宫和壶腹输卵管液（交配后11小时，即，排卵后1小时）、猴输卵管液、牛肾上腺皮质“运动因子”制剂和人、豚鼠和仓鼠精子制剂。基于这些结果，我们建议的可能性，牛磺酸和亚牛磺酸可能有作用，在体内的维持和刺激精子活力和刺激获能和/或顶体反应。
Previous work from this laboratory has shown that the β‐amino acid taurine can support and stimulate hamster sperm motility during in vitro capacitation in the presence or absence of epinephrine. The present report describes in vitro results which demonstrate that hypotaurine, a precursor of taurine, can also support and stimulate motility under these conditions and that a higher number of acrosome reactions occur in the presence of taurine as compared to hypotaurine (both in the presence and absence of epinephrine). In all cases, the greates percentage of acrosome reactions occurs in the presence of epinephrine. Whether these β‐amino acids act independently of epinephrine of in a synergistic manner with it remains to be determined. In addition to these in vitro studies, we report that hypotaurine and taurine are present at high levels in bovine follicular fluid, rabbit uterine and ampullar oviductal fluid (11 hr after mating, i.e., 1 hr after ovulation), monkey oviductal fluid, bovine adrenal cortex “motility factor” preparation and human, guinea pig and hamster sperm preparations. Based on these results, we suggest the possibility that taurine and hypotaurine may have roles in vivo in the maintenance and stimulation of sperm motility and stimulation of capacitation and/or acrosome reactions.