Mapping the location of TFIIB within the RNA polymerase II transcription preinitiation complex: A model for the structure of PIC
Mapping the location of TFIIB within the RNA polymerase II transcription preinitiation complex: A model for the structure of PIC
复制标题
DOI：
10.1016/j.cell.2004.09.028
复制
发表时间：
2004-10-15
期刊：
影响因子：
64.5
通讯作者：
Hahn, S
中科院分区：
文献类型：
--
作者：
Chen, HT;Hahn, S
位于一般转录因子TFIIB表面的生化探针被用来探测RNA聚合酶II（Pol II）转录前起始复合物（PIC）的结构。在PIC中，TFIIB接头和核心结构域位于Pol II的中央裂隙和壁上方。在较小的Pol II-TFIIB复合物中未观察到这种定位。这些结果导致了一个新的模型的PIC的结构，这与大多数以前记录的蛋白质-DNA相互作用内Pol II和古细菌PIC。TFIIB核心结构域与Pol II的特异性相互作用使启动子DNA定位并定向在Pol II中央裂缝上，并且TBP-DNA弯曲导致启动子在Pol II表面周围弯曲。发现TFIIF亚基Tfg 1与TFIIB B指、接头和核心结构域非常接近，表明这两个因子在起始过程中密切合作。
Biochemical probes positioned on the surface of the general transcription factor TFIIB were used to probe the architecture of the RNA polymerase II (Pol II) transcription preinitiation complex (PIC). In PICs, the TFIIB linker and core domains are positioned over the central cleft and wall of Pol II. This positioning is not observed in the smaller Pol II-TFIIB complex. These results lead to a new model for the structure of the PIC, which agrees with most previously documented protein-DNA interactions within Pol II and archaea PICs. Specific interaction of the TFIIB core domain with Pol II positions and orients the promoter DNA over the Pol II central cleft, and TBP-DNA bending leads to bending of the promoter around the surface of Pol II. The TFIIF subunit Tfg1 was found in close proximity to the TFIIB B finger, linker, and core domains, suggesting that these two factors closely cooperate during initiation.