Evidence for association between endothelial nitric oxide synthase gene polymorphism (G894T) and inflammatory markers: The ATTICA study
Evidence for association between endothelial nitric oxide synthase gene polymorphism (G894T) and inflammatory markers: The ATTICA study
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DOI：
10.1016/j.ahj.2004.04.022
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发表时间：
2004-10-01
影响因子：
4.8
通讯作者：
Stefanadis, C
中科院分区：
文献类型：
--
作者：
Chrysohoou, C;Panagiotakos, DB;Stefanadis, C
背景我们评估了内皮一氧化氮合酶（eNOS）基因第894位核苷酸（G894T）鸟嘌呤点突变为胸腺嘧啶对炎症和氧化应激标志物的影响。方法我们研究了270名男性（18-87岁）和325名女性（18-89岁）的遗传信息。根据雅典大区的年龄-性别分布，从普通人群中随机选择没有任何心血管或其他动脉粥样硬化疾病临床证据的参与者。使用标准方法从2至5mL新鲜或冷冻全血中提取基因组DNA。结果DNA分析显示10.6%的参与者是Asp纯合子(Asp/Asp)、40%杂合子(Asp/Glu)和49.4%Glu纯合子(Glu/Glu)。与 Asp/Glu 和 Glu/Glu 相比，Asp/Asp 具有更高水平的纤维蛋白原（332 +/- 46 或 329 +/- 33 vs 319 +/- 29 mg/dL，P = 0.029）、白细胞计数（6.9 +/- 0.6 或 6.5 +/- 0.3 vs 6.1 +/- 0.9 X 10(3) 计数，P =.044），以及氧化低密度脂蛋白胆固醇（68 +/- 21 或 61 +/- 22 vs 59 +/- 20 mg/dL，P =.039），在控制了几个潜在的混杂因素后。发现同型半胱氨酸 (P = .08)、C 反应蛋白 (P = .096) 和 G894T 多态性分布 (P
Background We evaluated the effect of,the point mutation of guanine to thymine at nucleotide position 894 (G894T) of the endothelial nitric oxide synthase (eNOS) gene on inflammatory and oxidative stress markers.Methods We studied genetic information from 270 men (18-87 years old) and 325 women (18-89 years old). Participants without any clinical evidence of cardiovascular or other atherosclerotic disease were randomly selected from the general population according to the age-sex distribution of Athens greater area. Genomic DNA was extracted from 2 to 5 mL of fresh or frozen whole blood using standard methods.Results The DNA analysis showed that 10.6% of the participants were Asp-homozygotes (Asp/Asp), 40% heterozygotes (Asp/Glu) and 49.4% Glu-homozygotes (Glu/Glu). Compared to Asp/Glu and Glu/Glu, Asp/Asp had higher levels of fibrinogen (332 +/- 46 or 329 +/- 33 vs 319 +/- 29 mg/dL, P =.029), white blood cells (6.9 +/- 0.6 or 6.5 +/- 0.3 vs 6.1 +/- 0.9 X 10(3) counts, P =.044), and oxidized low-density lipoprotein cholesterol (68 +/- 21 or 61 +/- 22 vs 59 +/- 20 mg/dL, P =.039), after controlling for several potential confounders. An insignificant association was found between homocysteine (P =.08), C-reactive protein (P =.096), and the distribution of G894T polymorphism (P