Bacterial attachment on titanium surfaces is dependent on topography and chemical changes induced by nonthermal atmospheric pressure plasma
Bacterial attachment on titanium surfaces is dependent on topography and chemical changes induced by nonthermal atmospheric pressure plasma
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DOI：
10.1088/1748-605x/aa734e
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发表时间：
2017-08-25
影响因子：
4
通讯作者：
Kim, Kwang-Mahn
中科院分区：
文献类型：
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作者：
Jeong, Won-Seok;Kwon, Jae-Sung;Kim, Kwang-Mahn
在这里，我们研究了非热大气压等离子体(NTAPP)引起的化学变化对光滑和粗糙钛的抗菌作用。用扫描电子显微镜观察了钛的光滑表面和粗糙表面的形貌。两种钛样品均在氮气气氛下用NTAPP处理10min。分别用光学轮廓仪、X射线光电子能谱和水接触角分析了表面粗糙度、化学成分和润湿性。通过测定菌落形成单位数和扫描电子显微镜分析来检测细菌的附着。粗糙的钛表面有不规则的微坑，而光滑的钛表面有相对规则的图案。NTAPP处理前后的标本在形态上无明显差异。NTAPP处理使粗糙和光滑的钛由疏水转变为亲水；粗糙的钛表现出相对较高的亲水性。在NTAPP处理前，血链球菌(S.sanguinis)在粗钛表面表现出较强的附着，而在NTAPP处理后，细菌附着显著减少。此外，细菌在粗糙钛上的附着率显著降低，而经NTAPP处理的钛上血链球菌菌落的结构发生了显著变化。无论表面形貌如何，NTAPP处理均可抑制钛种植体周围的细菌附着。因此，钛表面的NTAPP治疗是骨科和牙科领域应用抗菌治疗的下一代工具。
Here, we investigated the antibacterial effects of chemical changes induced by nonthermal atmospheric pressure plasma (NTAPP) on smooth and rough Ti. The morphologies of smooth and rough surfaces of Ti were examined using scanning electron microscopy (SEM). Both Ti specimens were then treated for 10 min by NTAPP with nitrogen gas. The surface roughness, chemistry, and wettability were examined by optical profilometry, x-ray photoelectron spectroscopy, and water contact angle analysis, respectively. Bacterial attachment was measured by determining the number of colony forming units and by SEM analysis. The rough Ti showed irregular micropits, whereas smooth Ti had a relatively regular pattern on the surface. There were no differences in morphology between samples before and after NTAPP treatment. NTAPP treatment resulted in changes from hydrophobic to hydrophilic properties on rough and smooth Ti; rough Ti showed relatively higher hydrophilicity. Before NTAPP treatment, Streptococcus sanguinis (S. sanguinis) showed greater attachment on rough Ti, and after NTAPP treatment, there was a significant reduction in bacterial attachment. Moreover, the bacterial attachment rate was significantly lower on rough Ti, and the structure of S. sanguinis colonies were significantly changed on NTAPP-treated Ti. NTAPP treatment inhibited bacterial attachment surrounding titanium implants, regardless of surface topography. Therefore, NTAPP treatment on Ti is a next-generation tool for antibacterial applications in the orthopaedic and dental fields.