Polyaniline stabilized Silver (I) Oxide nanocubes for sensitive and selective detection of hemoglobin in urine for hematuria evaluation
Polyaniline stabilized Silver (I) Oxide nanocubes for sensitive and selective detection of hemoglobin in urine for hematuria evaluation
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DOI：
10.1016/j.microc.2020.104723
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发表时间：
2020-06
影响因子：
4.8
通讯作者：
N. Swaminathan;Yowan Nerthigan;Hui-Fen Wu
中科院分区：
文献类型：
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作者：
N. Swaminathan;Yowan Nerthigan;Hui-Fen Wu
尿中带血或血尿已被视为多种泌尿系统疾病的征兆。一些血尿的诊断可能会给出假阳性结果，特别是在微量血尿的情况下。基于纳米材料的生物传感器已成为血尿分析的有效方法，可以作为尿液试纸法的可行替代方法。在本报告中，Ag2O纳米立方体是通过共沉淀法合成的，并用聚苯胺（PANI）进一步稳定。 PANI 稳定的 Ag2O 纳米立方体与血红蛋白 (Hb) 的血红素基团之间形成的生物复合物导致 Ag2O 纳米立方体在 420 nm 处的吸收强度增加。这是由于电子从血红素转变为 Ag+。这也会导致 Hb 的构象变化。 Ag2O-PANI 相互作用和稳定性研究通过紫外可见光谱 (UV-Vis)、傅里叶变换红外光谱 (FTIR)、X 射线衍射 (XRD) 和拉曼光谱得到证实。 Ag2O 纳米立方体的形貌通过透射电子显微镜 (TEM) 得到证实。紫外-可见光谱证实了 Ag2O-PANI 和 Hb 之间的相互作用。目前的检测对血尿非常敏感，尿液中的血尿检测限极低，为 5.35 × 10−13M。
Blood in urine or hematuria has been as an indication of a plethora of urological disorders. Some diagnoses of hematuria may give false positive results, especially in the cases of microhematuria. Nanomaterials based biosensors have been emerged as an effective method for hematuria analysis, which could serve as a viable alternative to urine dipstick method. In this report, the Ag2O nanocubes were synthesized by the co-precipitation method and further stabilized with polyaniline (PANI). The bio-complex formed between PANI stabilized Ag2O nanocubes, and heme group of Hemoglobin (Hb) resulted in an increase of absorption intensity of Ag2O nanocubes at 420 nm. This is due to the electronic transition from Heme to Ag+. This also leads to conformational changes in Hb. Ag2O-PANI interactions and stability studies were confirmed by Ultraviolet-Visible spectroscopy (UV–Vis), Fourier-Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD) and Raman spectroscopy. The morphology of Ag2O nanocubes was confirmed by Transmission electron Microscopy (TEM). The interaction between Ag2O-PANI and Hb was confirmed by UV–Vis spectroscopy. The current assay is very sensitive for hematuria with an extremely low detection limit of 5.35 × 10−13M from urine.