Isotachophoretic separations on a microchip. Normal Raman spectroscopy detection.
Isotachophoretic separations on a microchip. Normal Raman spectroscopy detection.
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DOI：
10.1021/ac980195h
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发表时间：
1998-08
影响因子：
7.4
通讯作者：
P. A. Walker;M. Morris;M. Burns;B. Johnson
中科院分区：
文献类型：
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作者：
P. A. Walker;M. Morris;M. Burns;B. Johnson
除草剂百草枯和敌草快的等速电泳分离在玻璃微芯片蚀刻通道中进行，并通过正常的拉曼光谱在芯片上进行监测。40微米宽和75微米深的分离通道以蛇形设计进行化学蚀刻，总长度为21厘米。使用120微米厚的玻璃盖片密封通道。分离场强可达380 V/cm。微芯片直接耦合到拉曼微探针。不需要接口。拉曼光谱是用2-W，532-nm NdY-VO 4激光器产生的，并用全息透射光谱仪和低温冷却CCD以8-cm-1分辨率收集。数据采集速度为2-5光谱/秒。本文提供了农药在低至2.3 x 10（-7）M（60 ppb百草枯/80 ppb敌草快）的起始浓度下的拉曼等速图。
Isotachophoretic separations of the herbicides paraquat and diquat are performed in a glass microchip etched channel and monitored on-chip by normal Raman spectroscopy. The 40-micron-wide and 75-micron-deep separation channels are chemically etched in a serpentine design to 21-cm total length. A 120-micron-thick glass cover slip is used to seal the channels. Separation field strengths up to 380 V/cm are used. The microchip is directly coupled to a Raman microprobe. No interfacing is required. Raman spectra are generated with a 2-W, 532-nm NdY-VO4 laser and collected at 8-cm-1 resolution with a holographic transmissive spectrograph and a cryogenically cooled CCD. Data acquisition is at 2-5 spectra/s. Raman isotachopherograms of the pesticides at starting concentrations as low as 2.3 x 10(-7) M (60 ppb paraquat/80 ppb diquat) are presented.