Differential Effects of NMDA-Induced Lesions into the Insular Cortex and Amygdala on the Acquisition and Evocation of Conditioned Immunosuppression
Differential Effects of NMDA-Induced Lesions into the Insular Cortex and Amygdala on the Acquisition and Evocation of Conditioned Immunosuppression
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DOI：
10.1006/brbi.1998.0518
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发表时间：
1998-06
期刊：
影响因子：
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通讯作者：
V. Ramírez-Amaya;Benjamı́n Alvarez-Borda;F. Bermúdez-Rattoni
中科院分区：
文献类型：
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作者：
V. Ramírez-Amaya;Benjamı́n Alvarez-Borda;F. Bermúdez-Rattoni
它已被确定，岛叶皮层（IC）介导的条件性味觉厌恶，最近我们已经证明，这种结构的病变破坏收购条件性免疫抑制（CIS）。IC与杏仁核（AM）在功能上和神经学上相互联系，已被认为参与神经免疫相互作用。这项工作的目的是测试NMDA诱导的病变的影响，无论是在IC或AM的收购（病变前的条件）和唤起（病变后的条件）的免疫抑制任务，获得一个单一的配对糖精的味道和免疫抑制药物，环磷酰胺。将AM和IC损伤大鼠分为4组：前2组在获得条件免疫抑制之前进行损伤，另2组在获得条件免疫抑制之后进行损伤。调节后20天，将动物再次暴露于糖精并用卵清蛋白免疫。免疫后，采集血样，并通过ELISA分析。结果表明，IC病变扰乱了CTA和CIS的获取和唤起。相反，AM病变仅破坏CIS的获取。这些数据表明，IC参与的神经机制的收购和唤起的条件性免疫抑制，和杏仁核可能是重要的介导输入的免疫信息收购条件性免疫抑制。
It has been established that the insular cortex (IC) mediates conditioned taste aversion, and recently we have demonstrated that lesions of this structure disrupt the acquisition of conditioned immunosuppression (CIS). The IC is functionally and reciprocally interconnected with the amygdala (AM) which has been suggested to be involved in neural-immune interactions. The aim of this work was to test the effects of NMDA-induced lesions in either the IC or AM in the acquisition (lesions made before conditioning) and evocation (lesions made after conditioning) of a conditioned immunosuppression task, obtained by one single pairing of saccharin taste and the immunosuppressive drug, cyclophosphamide. AM and IC lesioned rats were separated into four groups: the first two received lesions before and the other two were lesioned after the acquisition of conditioned immunosuppression. Twenty days after conditioning, animals were reexposed to saccharin and immunized with ovalbumin. After immunization, blood samples were taken, and analyzed by ELISA. The results showed that IC lesions disrupted the acquisition and evocation of CTA and CIS. Conversely, AM lesions disrupted only the acquisition of CIS. These data suggest that the IC is involved in the neural mechanisms underlying the acquisition and evocation of conditioned immunosuppression, and the amygdala could be important in mediating the input of the immune information necessary for the acquisition of conditioned immunosuppression.