CLA4 regulates leaf angle through multiple hormone signaling pathways in maize
CLA4 regulates leaf angle through multiple hormone signaling pathways in maize
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CLA4 通过多种激素信号通路调节玉米叶角
DOI：
10.1093/jxb/eraa565
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发表时间：
2021-02-27
影响因子：
6.9
通讯作者：
Chen, Yanhui
中科院分区：
文献类型：
--
作者：
Dou, Dandan;Han, Shengbo;Chen, Yanhui
叶角是禾谷类作物的重要农艺性状，与作物结构和产量关系密切。虽然之前已经报道了ZmCLA 4可以影响叶片角度，但其潜在机制仍不清楚。在这项研究中，我们使用Gal 4-莱克萨/UAS系统和反式激活分析，以证明在玉米（玉米），ZmCLA 4是一个转录抑制因子，调节叶角。DNA亲和纯化测序（DAP-Seq）分析显示，ZmCLA 4主要与含有启动子的启动子（CACCGGAC）以及另外两个基序（CCGARGS和CDTCNTC）结合以抑制其靶基因的表达。对ZmCLA 4靶基因的进一步分析表明，ZmCLA 4作为多种植物激素信号传导途径的枢纽发挥作用：ZmCLA 4被发现直接结合多个基因的启动子，包括生长素转运途径中的ZmARF 22和ZmIAA 26、油菜素类固醇信号途径中的ZmBZR 3、参与脱落酸代谢的两个ZmWRKY基因、ZmCYP基因（ZmCYP 75 B1，ZmCYP 93 D1）参与茉莉酸代谢，ZmABI 3参与乙烯反应途径。总的来说，我们的工作提供了深入了解ZmCLA 4调控网络在控制玉米叶角。
Leaf angle is an important agronomic trait in cereals and shares a close relationship with crop architecture and grain yield. Although it has been previously reported that ZmCLA4 can influence leaf angle, the underlying mechanism remains unclear. In this study, we used the Gal4-LexA/UAS system and transactivation analysis to demonstrate in maize (Zea mays) that ZmCLA4 is a transcriptional repressor that regulates leaf angle. DNA affinity purification sequencing (DAP-Seq) analysis revealed that ZmCLA4 mainly binds to promoters containing the EAR motif (CACCGGAC) as well as to two other motifs (CCGARGS and CDTCNTC) to inhibit the expression of its target genes. Further analysis of ZmCLA4 target genes indicated that ZmCLA4 functions as a hub of multiple plant hormone signaling pathways: ZmCLA4 was found to directly bind to the promoters of multiple genes including ZmARF22 and ZmIAA26 in the auxin transport pathway, ZmBZR3 in the brassinosteroid signaling pathway, two ZmWRKY genes involved in abscisic acid metabolism, ZmCYP genes (ZmCYP75B1, ZmCYP93D1) related to jasmonic acid metabolism, and ZmABI3 involved in the ethylene response pathway. Overall, our work provides deep insights into the ZmCLA4 regulatory network in controlling leaf angle in maize.