Autoradiographic localization of somatostatin receptors in rat brain.
Autoradiographic localization of somatostatin receptors in rat brain.
复制标题
大鼠脑中生长抑素受体的放射自显影定位。
DOI：
10.1016/0014-2999(84)90178-x
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发表时间：
1984
影响因子：
5
通讯作者：
Snyder,SH
中科院分区：
文献类型：
--
作者：
Tran,VT;Uhl,GR;Perry,DC;Manning,DC;Vale,WW;Perrin,MH;Rivier,JE;Martin,JB;Snyder,SH
生长抑素 (SS) 与神经内分泌、运动和高级皮质脑功能有关 (Reichlin, 1983)。阿尔茨海默病和帕金森痴呆症中皮质 SS 水平选择性降低，而亨廷顿病中纹状体浓度升高（Reichlin，1983；Epelbaum 等，1983）。放射自显影可视化可能允许对正常和患病大脑中的 SS 受体进行表征，但配体的亲脂性和不稳定性阻碍了这种努力。由稳定且亲水的类似物（例如 [Leu8-D-Trp 22, Tyr25] SS-28 (LTT)）促进的结合研究已表征出具有皮摩尔亲和力的脑 SS 受体（Reubi 等人，1981；Perry 等人，1983）。现在，我们使用 125I-LTT 和 125I-Tyr11SS 报告了大鼠大脑中 SS 受体位点的放射自显影可视化。戊巴比妥麻醉的大鼠心内灌注0.1M磷酸钠缓冲液中的0.32M蔗糖，pH 7.4。将12μm低温恒温器切片解冻到铺有垫片的载玻片上。将约 0.16 nM~ 25I-Tyr~ 1SS 或 0.04 nM 125I-LTT 与切片一起在 150 mM Tris 缓冲液（pH 7.4，含有 0.1% 杆菌肽、0.1% 牛血清白蛋白和 5 mM MgSO 4 ）中于 25°C 孵育 1 小时。使用 2 x 10-7 M TyrllSS 进行平行孵育。作为空白。将切片在 4 o C 下于 150 mM Tris（pH 7.4）中清洗 3 次，每次 15 分钟，快速干燥并涂上乳液
Somatostatin (SS) is implicated in neuroendocrine, motor, and higher cortical brain functions (Reichlin, 1983). Cortical SS levels are selectively reduced in Alzheimer's disease and Parkinsonian dementia, while striatal concentrations are increased in Huntington's disease (Reichlin, 1983; Epelbaum et al., 1983). Autoradiographic visualization may permit SS receptor characterization in normal and diseased brain but lipophilicity and instability of ligands has hampered such efforts. Binding studies facilitated by stable and hydrophilic analogs such as [Leu8-D-Trp 22, Tyr25] SS-28 (LTT), have characterized brain SS receptors with picomolar affinity (Reubi et al., 1981; Perry et al., 1983). Using 125I-LTT as well as 125I-Tyr11SS we now report the autoradiographic visualization of SS receptor sites in rat brain. Pentobarbital-anesthesized rats were perfused intracardially with 0.32 M sucrose in 0.1 M sodium phosphate buffer, pH 7.4 Twelve~ m cryostat sections were thaw-mounted onto subbed slides. Approximately 0.16 nM~ 25I-Tyr~ 1SS or 0.04 nM 125I-LTT was incubated with sections in 150 mM Tris buffer, pH 7.4 containing 0.1% bacitracin, 0.1% bovine serum albumin and 5 mM MgSO 4 for 1 h at 25 C. Parallel incubations utilized 2 x 10-7 M TyrllSS as a blank. Sections were washed three times for 15 min each in 150 mM Tris, pH 7.4, at 4 o C, rapidly dried and apposed to emulsion-coated