Sec3p is involved in secretion and morphogenesis in Saccharomyces cerevisiae
Sec3p is involved in secretion and morphogenesis in Saccharomyces cerevisiae
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DOI：
10.1091/mbc.8.4.647
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发表时间：
1997-04-01
影响因子：
3.3
通讯作者：
Novick, P
中科院分区：
文献类型：
--
作者：
Finger, FP;Novick, P
两个新的温度敏感的等位基因的SEC 3，1的10个迟效SEC基因所需的后高尔基体分泌囊泡的质膜在酿酒酵母中的靶向或融合，分离在一个屏幕上的温度敏感的分泌突变体，是合成致命的sec 4 -8。新的sec 3等位基因影响分泌的早期和晚期。SEC 3基因的克隆和测序表明，它是相同的profilin合成致死1（PSL 1）。SEC 3基因不是必需的，因为耗尽Sec 3 p的细胞是存活的，尽管生长缓慢且对温度敏感。所有的sec 3等位基因在遗传上都与profilin突变pfy 1 -111相互作用。高拷贝的SEC 3基因抑制pfy 1 -111和sec 5 -24，并导致ypt 1、sec 8 -9、sec 10 -2和sec 15 -1的合成生长缺陷。肌动蛋白结构仅在Sec 3 p功能慢性丧失的情况下受到干扰，这意味着Sec 3 p不直接调节肌动蛋白。在纯合二倍体中，sec 3的所有等位基因导致芽位选择缺陷，sec 4 -8和sec 9 -4也是如此。这表明SEC基因产物参与确定芽位点，并且与Sec 3 p在确定胞吐的正确位点中的作用一致。
Two new temperature-sensitive alleles of SEC3, 1 of 10 late-acting SEC genes required for targeting or fusion of post-Golgi secretory vesicles to the plasma membrane in Saccharomyces cerevisiae, were isolated in a screen for temperature-sensitive secretory mutants that are synthetically lethal with sec4-8. The new sec3 alleles affect early as well as late stages of secretion. Cloning and sequencing of the SEC3 gene revealed that it is identical to profilin synthetic lethal 1 (PSL1). The SEC3 gene is not essential because cells depleted of Sec3p are viable although slow growing and temperature sensitive. All of the sec3 alleles genetically interact with a profilin mutation, pfy1-111. The SEC3 gene in high copy suppresses pfy1-111 and sec5-24 and causes synthetic growth defects with ypt1, sec8-9, sec10-2, and sec15-1. Actin structure is only perturbed in conditions of chronic loss of Sec3p function, implying that Sec3p does not directly regulate actin. All alleles of sec3 cause bud site selection defects in homozygous diploids, as do sec4-8 and sec9-4. This suggests that SEC gene products are involved in determining the bud site and is consistent with a role for Sec3p in determining the correct site of exocytosis.