Morphological characterization of GFP stably transfected adult mesenchymal bone marrow stem cells
Morphological characterization of GFP stably transfected adult mesenchymal bone marrow stem cells
复制标题
DOI：
10.1111/j.1469-7580.2006.00511.x
复制
发表时间：
2006-01-01
影响因子：
2.4
通讯作者：
Geuna, S
中科院分区：
文献类型：
--
作者：
Raimondo, S;Penna, C;Geuna, S
越来越多的注意力被给予成人而不是胚胎干细胞的使用，无论是用于研究还是用于开发人类疾病的移植治疗。特别是，间充质骨髓干细胞已经被广泛研究，因为它们具有自我更新和产生各种分化细胞类型的能力，并且因为它们可以相对容易地获得和培养。此外，在移植前用绿色荧光蛋白标记干细胞的可能性为干细胞在基础研究中的应用开辟了新的有希望的前景。由于没有结构或超微结构的描述，成人间充质干细胞是在文献中，本文描述了它们的形态学所揭示的光，共聚焦和电子显微镜，专注于细胞，特别适合移植研究，即那些来自大鼠骨髓转染绿色荧光蛋白。这一结果为研究这些细胞在正常组织和病理组织中的分化提供了实验依据。
Increasing attention is being given to the use of adult rather than embryonic stem cells, both for research and for the development of transplantation treatments for human disease. In particular, mesenchymal bone marrow stem cells have been studied extensively because of their ability to self-renew and to give rise to various differentiated cell types, and because of the relative ease with which they can be obtained and cultured. In addition, the possibility of labelling stem cells with green fluorescent protein before transplantation has opened new and promising perspectives for their use in basic research. Because no structural or ultrastructural description of adult mesenchymal stem cells is available in the literature, this paper describes their morphology as revealed by light, confocal and electron microscopy, focusing on cells that are particularly suitable for transplantation studies, i.e. those derived from rat bone marrow transfected with green fluorescent protein. The results provide a basis for experimental studies of the differentiation of these cells in normal and pathological tissues.