DNA multilayer film for loading and release of DNA oligomer
DNA multilayer film for loading and release of DNA oligomer
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用于装载和释放 DNA 寡聚物的 DNA 多层膜
DOI：
10.1051/matecconf/20179802001
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发表时间：
2017
影响因子：
--
通讯作者：
N. Kato
中科院分区：
文献类型：
--
作者：
Y. Kamimura;N. Kato
使用聚电解质的逐层（LbL）方法是制备功能性聚合物多层膜的典型策略。除了带正电和带负电的聚电解质之间的静电力外，互补 DNA 链之间的杂交力也可用于构建 DNA LbL 薄膜。在此，我们报道了热响应 DNA LbL 薄膜，该薄膜旨在在高温下释放特定的 DNA 寡聚物（10 个碱基）。由于介质的温度和离子强度 (IS) 会影响双链 DNA 中的氢键，因此研究了薄膜对温度和离子强度的结构稳定性。在 DNA LbL 膜上重复进行可释放低聚物的加载和释放。
The Layer-by-Layer (LbL) method using polyelectrolytes is a typical strategy for preparing functional polymeric multi-layered films. Besides the electrostatic force between positively and negatively charged polyelectrolytes, the hybridization force between complementary DNA strands can be also used for building the DNA LbL films. Herein, we report the thermal responsible DNA LbL film that is designed to release specific DNA oligomers (10 bases) at an elevated temperature. Because the temperature and the ionic strength (IS) of the medium affect the hydrogen bonds in the double stranded DNA, the structural stability of the film against temperature and IS was investigated. The loading and release of the releasable oligomers were repeatedly performed on the DNA LbL film.