In vivo MRS measurement of liver lipid levels in mice
In vivo MRS measurement of liver lipid levels in mice
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DOI：
10.1194/jlr.d400001-jlr200
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发表时间：
2004-07-01
影响因子：
6.5
通讯作者：
Schonfeld, G
中科院分区：
文献类型：
--
作者：
Garbow, JR;Lin, X;Schonfeld, G
磁共振波谱（MRS）程序在体内测量小鼠肝脏中的脂质水平的描述和验证。该方法使用质子门控，局部光谱学收集小鼠肝脏内的体素的质子光谱。这些光谱的贝叶斯概率理论分析允许精确测量肝脏内脂质和水共振的相对强度。所有的光谱数据进行了校正测量自旋-自旋弛豫。本研究共使用了48只小鼠，包括野生型小鼠和两种不同的转基因小鼠品系。这些小鼠中的不同组被喂食高脂肪或低脂肪饮食或添加或不添加酒精的流质饮食。在基线时收集质子谱，随后每4周收集一次，持续16周。最后一次MRS测量后，立即杀死小鼠，并通过常规湿化学方法分析其肝脏的甘油三酯水平。肝脏脂质的体内MRS和离体湿化学测定之间的良好相关性验证了MRS方法。这些结果清楚地表明，体内MRS将是一种非常有价值的纵向研究技术，旨在为影响小鼠肝脏内脂肪沉积和积累的遗传，环境和饮食因素提供重要见解。加博，J.R.，X. Lin，N. Sakata，Z. Chen，中国粘蝇D. Koh和G.舍恩菲尔德小鼠肝脏脂质水平的体内MRS测量。
A magnetic resonance spectroscopy (MRS) procedure for in vivo measurement of lipid levels in mouse liver is described and validated. The method uses respiratory-gated, localized spectroscopy to collect proton spectra from voxels within the mouse liver. Bayesian probability theory analysis of these spectra allows the relative intensities of the lipid and water resonances within the liver to be accurately measured. All spectral data were corrected for measured spin-spin relaxation. A total of 48 mice were used in this study, including wild-type mice and two different transgenic mouse strains. Different groups of these mice were fed high-fat or low-fat diets or liquid diets with and without the addition of alcohol. Proton spectra were collected at baseline and, subsequently, every 4 weeks for up to 16 weeks. Immediately after the last MRS measurement, mice were killed and their livers analyzed for triglyceride level by conventional wet-chemistry methods. The excellent correlation between in vivo MRS and ex vivo wet-chemistry determinations of liver lipids validates the MRS method. These results clearly demonstrate that in vivo MRS will be an extremely valuable technique for longitudinal studies aimed at providing important insights into the genetic, environmental, and dietary factors affecting fat deposition and accumulation within the mouse liver.-Garbow, J. R., X. Lin, N. Sakata, Z. Chen, D. Koh, and G. Schonfeld. In vivo MRS measurement of liver lipid levels in mice.