Experience-dependent formation of activity propagation patterns at the somatosensory S1 and S2 boundary in rat cortical slices
Experience-dependent formation of activity propagation patterns at the somatosensory S1 and S2 boundary in rat cortical slices
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DOI：
10.1016/j.neuroimage.2006.08.049
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发表时间：
2007-03-01
期刊：
影响因子：
5.7
通讯作者：
Shibuki, Katsuei
中科院分区：
文献类型：
--
作者：
Kamatani, Dalki;Hishida, Ryuichi;Shibuki, Katsuei
躯体感觉信息由初级（S1）和次级（S2）皮层连续处理，这可以在新鲜的皮层切片中识别。我们可视化活动之间的传播S1和S2在大鼠皮质切片黄素蛋白荧光成像。当刺激S1时，荧光反应延伸到S2，而S2刺激后的反应几乎不传播到S1。从S1到S2的优势活动传播模式不受谷氨酸或GABA（A）受体拮抗剂的影响。Ca 2+成像和电生理记录证实了各向异性活动传播模式。该图案可以作为S1和S2中的串行信息处理的结果而形成。为了验证这一假设，在修剪对侧须后2周或3天制备的皮质切片中研究了活动传播，所述对侧须为SI提供大量输入。在桶皮质的颗粒上的活动明显受到抑制。此外，在S1和S2之间的边界附近的S1的吻侧小触须/口区引起的活动扩散到相邻的桶皮质，而不是进入S2。使用测试来评估胡须修剪的行为效果，在测试中，大鼠选择仅在右侧或左侧有墙的两个桥中的一个。在单侧胡须修剪后，大鼠更喜欢使用靠近完整侧的桥。然而，这种偏好在修剪后3天消失。修饰后的活动观察到的皮层切片胡须修剪可能是这种行为补偿的机制。这些研究结果表明，经验依赖的形成活动传播模式的躯体感觉皮层。(c)2006年爱思唯尔公司All rights reserved.
Somatosensory information is serially processed by the primary (S1) and secondary (S2) cortices, which can be identified in fresh cortical slices. We visualized activity propagation between S1 and S2 in rat cortical slices using flavoprotein fluorescence imaging. When S1 was stimulated, fluorescence responses extended into S2, while responses hardly propagated to S1 following S2 stimulation. The dominant activity propagation pattern from S1 to S2 was not affected by antagonists of glutamate or GABA(A) receptors. Ca2+ imaging and electrophysiological recordings confirmed the anisotropic activity propagation pattern. This pattern could be formed as a result of serial information processing in SI and S2. To test this hypothesis, activity propagation was investigated in cortical slices prepared 2 weeks or 3 days after trimming contralateral whiskers that provide massive inputs to SI. Supragranular activities in the barrel cortex were clearly suppressed. Furthermore, activities elicited in the rostral small vibrissae/mouth area of S1 near the border between S I and S2 spread into the adjacent barrel cortex rather than into S2. Behavioral effects of whisker trimming were evaluated using a test, in which rats chose one of two bridges that had a wall on the right or left side only. Immediately after hemilateral whisker trimming, rats preferred to use the bridge with a wall close to the intact side. However, this preference disappeared 3 days after trimming. Modified activities observed in cortical slices after whisker trimming might be mechanisms for this behavioral compensation. These findings suggest experience-dependent formation of activity propagation patterns in the somatosensory cortex. (c) 2006 Elsevier Inc. All rights reserved.