SP1 TRANSCRIPTION FACTOR BINDS DNA AND ACTIVATES TRANSCRIPTION EVEN WHEN THE BINDING-SITE IS CPG METHYLATED
SP1 TRANSCRIPTION FACTOR BINDS DNA AND ACTIVATES TRANSCRIPTION EVEN WHEN THE BINDING-SITE IS CPG METHYLATED
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DOI：
10.1101/gad.2.9.1127
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发表时间：
1988-09-01
影响因子：
10.5
通讯作者：
SCHAFFNER, W
中科院分区：
文献类型：
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作者：
HOLLER, M;WESTIN, G;SCHAFFNER, W
在脊椎动物中，基因活性与DNA的CpG甲基化之间存在负相关，特别是在启动子区域。因此，可想而知，转录因子与甲基化和非甲基化结合位点的不同结合对基因活性至关重要。由于转录因子Sp1的共同结合部位含有一个中心CpG，我们研究了Sp1因子与未甲基化和人工合成的CpG甲基化DNA的结合。在识别序列的中心和外围的两个CpG位置，两条链上都有一个很强的Sp1结合位点甲基化。我们的研究表明，Sp1结合位点的甲基化既不影响体外结合，也不影响体内和体外转录。我们讨论了Sp1因子结合的可能性，它通常与管家基因的启动子相关，阻止CpG甲基化。
In vertebrates, a negative correlation between gene activity and CpG methylation of DNA, notably in the promoter region, is well established. Therefore, it is conceivable that differential binding of transcription factors to methylated versus unmethylated binding sites is crucial for gene activity. Since the consensus binding site of transcription factor Sp1 contains a central CpG, we have investigated the binding of Sp1 factor to unmethylated and synthetically CpG-methylated DNA. A strong Sp1 binding site was methylated on both strands at two CpG positions, located in the center and at the periphery of the recognition sequence. Our studies show that neither binding in vitro, nor transcription in vivo and in vitro are affected by methylation of the Sp1 binding site. We discuss the possibility that binding of Sp1 factor, which is often associated with promoters of housekeeping genes, prevents CpG methylation.