In vivo magnetic resonance imaging of iron oxide-labeled, arterially-injected mesenchymal stem cells in kidneys of rats with acute ischemic kidney injury:: Detection and monitoring at 3T
In vivo magnetic resonance imaging of iron oxide-labeled, arterially-injected mesenchymal stem cells in kidneys of rats with acute ischemic kidney injury:: Detection and monitoring at 3T
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DOI：
10.1002/jmri.20925
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发表时间：
2007-06-01
影响因子：
4.4
通讯作者：
Nolte-Ernsting, Claus
中科院分区：
文献类型：
--
作者：
Ittrich, Harald;Lange, Claudia;Nolte-Ernsting, Claus
目的：评估MRI对急性肾损伤（阿基）大鼠主动脉内注射的氧化铁标记间充质干细胞（MSC）的定性和定量体内追踪。材料和方法：对未标记和超顺磁性氧化铁（SPIO）标记的MSC（MSCSPIO）进行体外MRI和R-2* 测量，与细胞铁含量和细胞学检查（普鲁士蓝，电子显微镜）相关。在缺血/再灌注阿基（N = 14）和颅内注射1.5 × 10（6）MSCSPIO（N = 7）、胎牛血清（FCS）（培养基，N = 6）和SPIO单独给药（N = 1）之前和之后，使用临床3 T扫描仪进行体内MRI和R2* 评估，持续14天。信噪比（SNR），肾脏、肝脏、脾脏和骨髓的R2*，肾功能测量（肌酐[CREA]，血尿素氮[BUN]）和肾脏体积，并进行统计学显著性检验（Student t检验，P < 0.05）（苏木精和伊红[H&E]，普鲁士蓝，高碘酸-希夫[PAS]，CD 68）。在体外，MSCSPIO显示SNR和T-2* 降低，R-2* 接近MSCSPIO的数量（R-2 = 0.98）。与对照动物相比，体内MSCspIO给药导致肾皮质中由MSCSPIO蓄积引起的SNR降低（35 +/- 15%）和R-2* 增加（101 +/- 18.3%）（P < 0.01）。肝脏、脾脏和骨髓（MSCSPIO）显示延迟的SNR下降/R-2* 增加（P <0.05）。结论：在3 T MRI上对阿基大鼠腹腔内注射MSCSPIO进行定性、定量的活体细胞追踪和器官分布监测是可行的。
Purpose: To evaluate MRI for a qualitative and quantitative in vivo tracking of intraaortal injected iron oxide-labeled mesenchymal stem cells (MSC) into rats with acute kidney injury (AKI).Materials and Methods: In vitro MRI and R-2* measurement of nonlabeled and superparamagnetic iron oxide (SPIO)-labeled MSC (MSCSPIO) was performed in correlation, to cellular iron content and cytological examination (Prussian blue, electron microscopy). In vivo MRI and R2* evaluation were performed before and after ischemic/reperfusion AKI (N = 14) and intraaortal injection of 1.5 X 10(6) MSCSPIO (N = 7), fetal calf serum (FCS) (medium, N = 6), and SPIO alone (N = 1) up to 14 days using a clinical 3T scanner. Signal to noise ratios (SNR), R2* of kidneys, liver, spleen, and bone marrow, renal function (creatinine [CREA], blood urea nitrogen [BUN)), and kidney volume were measured and tested for statistical significance (Student's t-test, P < 0.05) in comparison histology (hematoxylin and eosin [H&E], Prussian blue, periodic acid-Schiff [PAS], CD68).Results: In vitro, MSCSPIO showed a reduction of SNR and T-2* with R-2* approximate to number of MSCSPIO) (R-2 = 0.98). In vivo MSCspIo administration resulted in a SNR decrease (35 +/- 15%) and R-2* increase (101 +/- 18.3%) in renal cortex caused by MSCSPIO accumulation in contrast to control animals, (P < 0.01). Liver, spleen, and bone marrow (MSCSPIO) showed a delayed SNR decline/R-2* increase (P < from MSCSPIO migration. The increase of kidney volume and the decrease in renal function (P < 0.05) was reduced in MSC-treated animals.Conclusion: Qualitative and quantitative in vivo cell-tracking and monitoring of organ distribution of intraaortal injected MSCSPIO in AKI is feasible in MRI at 3T.