Radioautographic analysis of 3H-fucose utilization by fibroblasts of the periodontal ligament.
Radioautographic analysis of 3H-fucose utilization by fibroblasts of the periodontal ligament.
复制标题
牙周膜成纤维细胞利用 3 H-岩藻糖的放射自显影分析。
DOI：
10.1111/j.1600-0765.1986.tb01439.x
复制
发表时间：
1986
影响因子：
3.5
通讯作者：
Garant,PR
中科院分区：
文献类型：
--
作者：
Cho,MI;Garant,PR
采用光镜和电镜放射自显影技术研究了牙周膜成纤维细胞分泌3种标记糖蛋白的情况。在5点，10点。静脉注射后20分钟，3 H-岩藻糖在高尔基体池和囊泡中浓缩。到35分钟时，标记物分散到细胞外周和细胞外基质。在注射后8小时，几乎所有的放射性标记物都与细胞表面或邻近的细胞外基质结合。标记的糖蛋白包含在胶原分泌颗粒中，并且似乎与胶原前体物质紧密相关。3 H-岩藻糖标记物质在牙周膜中均匀分布。
The secretion of3labeld glycoproteins from periodontal ligament fibroblasts was, studied by light and electron microseopic radioautography. At 5, 10. and 20 minutes after intravenous injection,3H‐fucose was concentrated in Golgi cisternae and saccules. By 35 minutes, the label was dispersed to the cell periphery and extracellular matrix. At 8 hours after injection, almost all of the radioactive label was associated with the cell surface or the adjacent extracellular matrix. Labeled glycoprotein was contained in collagen secretion granules and appeared to be relesasd simultaneousley with the collagen precursors The distribution of3H‐fucose labeled material was uniform across the periodontal ligament.