Snapshot of a DNA polymerase while incorporating two consecutive C5-modified nucleotides.
Snapshot of a DNA polymerase while incorporating two consecutive C5-modified nucleotides.
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DOI：
10.1021/ja405346s
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发表时间：
2013-10
影响因子：
15
通讯作者：
S. Obeid;H. Busskamp;W. Welte;K. Diederichs;A. Marx
中科院分区：
文献类型：
--
作者：
S. Obeid;H. Busskamp;W. Welte;K. Diederichs;A. Marx
功能性核苷酸在许多尖端生物分子技术中非常重要。通常必须连续掺入几个修饰的核苷酸。 KlenTaq DNA 聚合酶（一种水生栖热菌 DNA 聚合酶的截短形式）的结构研究首次揭示了 DNA 聚合酶如何处理多重修饰，因此有助于理解这些酶与人工底物的相互作用。
Functional nucleotides are important in many cutting-edge biomolecular techniques. Often several modified nucleotides have to be incorporated consecutively. This structural study of KlenTaq DNA polymerase, a truncated form of Thermus aquaticus DNA polymerase, gives first insights how multiple modifications are processed by a DNA polymerase and, therefore, contribute to the understanding of these enzymes in their interplay with artificial substrates.