Noxious heat receptors present in cold-sensory cells in rats
Noxious heat receptors present in cold-sensory cells in rats
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DOI：
10.1016/j.neulet.2004.01.074
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发表时间：
2004-04-08
影响因子：
2.5
通讯作者：
Kobayashi, S
中科院分区：
文献类型：
--
作者：
Okazawa, M;Inoue, W;Kobayashi, S
当夜间温度高于45 ℃时，冷点会激活冷纤维，从而引起一种反常的冷感觉。冷感受器是否对热也有反应，或者有毒的热感受器和冷感受器是否共存于同一纤维中，这一问题仍然没有解决。最近，已经克隆了有害热受体（TRPV1）和冷受体（TRPM8）。然而，TRPV1和TRPM8是否共存于同一个感觉神经元仍存在争议。在这里，我们调查共定位这些受体在背根神经节（DRG）的大鼠。在DRG切片中，TRPV1在29%的TRPM8阳性细胞中表达。在Ca2+成像中，有害的热刺激了培养中的许多冷敏感细胞。在全细胞电流钳模式下，有害热，辣椒素，冷却和薄荷醇都诱发了DRG神经元亚群的受体电位和冲动。TRPV1和TRPM8在DRG神经元中的共定位可能是反常冷感觉的基础。(C)2004爱思唯尔爱尔兰有限公司保留所有权利。
Noxious heat above similar to45 degreesC applied on cold spots evokes a paradoxical cold sensation by activating cold fibers. It remains unresolved whether cold receptors respond to heat as well, or whether noxious-heat receptors and cold receptors coexist in the same fiber. Recently, noxious heat receptors (TRPV1) and cold receptors (TRPM8) have been cloned. It is controversial, however, whether TRPV1 and TRPM8 coexist in the same sensory neuron. Here, we investigate colocalization of these receptors in dorsal root ganglion (DRG) of rats. TRPV1 was expressed in 29% of TRPM8-positive cells in DRG sections. In Ca2+-imaging, noxious heat excited many of the cold-sensitive cells in culture. In a whole-cell current-clamp mode, noxious heat, capsaicin, cooling and menthol all evoked receptor potentials and impulses in a subset of DRG neurons. This colocalization of TRPV1 and TRPM8 in a DRG neuron may be the basis for the paradoxical cold sensation. (C) 2004 Elsevier Ireland Ltd. All rights reserved.