Galectin-3 induced by hypoxia promotes cell migration in thyroid cancer cells.
Galectin-3 induced by hypoxia promotes cell migration in thyroid cancer cells.
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DOI：
10.18632/oncotarget.21135
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发表时间：
2017-11-24
期刊：
影响因子：
--
通讯作者：
Ai Z
中科院分区：
文献类型：
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作者：
Zheng J;Lu W;Wang C;Xing Y;Chen X;Ai Z
本研究的目的是探讨Galectin-3在人甲状腺癌转移中的作用。免疫组织化学和免疫印迹法检测Galectin-3在手术标本中的表达。将Galectin-3短发夹状RNA(Gal-3-shRNA)导入人乳头状甲状腺癌细胞系(B-CPAP)和间变性甲状腺癌细胞系(8305c)。低分子柑橘果胶(LCP)也被用来对抗Galectin-3。观察细胞的迁移和侵袭能力。通过对相关信号通路的研究，探讨Galectin-3的作用机制。Galectin-3在转移性甲状腺癌中高表达。下调和拮抗Galectin-3可显著抑制甲状腺癌细胞的迁移。Galectin-3基因敲除可抑制Wnt、MAPK、Src和Rho信号通路的活性。在低氧环境中，Galectin-3通过HIF-1α表达上调。Galectin-3基因敲除可降低细胞在低氧环境中的运动能力。这项研究表明Galectin-3可能作为甲状腺癌转移的调节器，特别是在低氧微环境中。Galectin-3的这种调节功能可能通过多条信号通路发挥作用。
The aim of this study is to investigate the role of Galectin-3 in human thyroid cancer migration. The expression of Galectin-3 in surgical specimens was investigated using immunohistochemistry and western blot. A papillary thyroid cancer cell line (B-cpap) and an anaplastic thyroid cancer cell line (8305c) were transfected with short-hairpin RNA against Galectin-3 (Gal-3-shRNA). Low-molecular citrus pectin (LCP) was also used to antagonize Galectin-3. The migration and invasion of the cell lines were examined. The related signaling pathways were investigated to explore the Galectin-3 mechanism of action. Galectin-3 was highly expressed in metastasized thyroid cancers. Knocking down and antagonizing Galectin-3 significantly suppressed the migration of thyroid cancer cells. Knocking down Galectin-3 inhibited the activity of Wnt, MAPK, Src and Rho signaling pathways. Galectin-3 was up-regulated via HIF-1α in a hypoxic environment. Galectin-3 knockdown could reduce cell motility in hypoxic environments. This study suggests that Galectin-3 could act as a modulator of thyroid cancer migration, especially in hypoxic microenvironments. This regulation function of Galectin-3 may work through multiple signaling pathways.