Isolation and characterization of mesoangioblasts from mouse, dog, and human tissues.
Isolation and characterization of mesoangioblasts from mouse, dog, and human tissues.
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DOI：
10.1002/9780470151808.sc02b01s3
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发表时间：
2007-12-01
影响因子：
--
通讯作者：
Sampaolesi, Maurilio
中科院分区：
文献类型：
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作者：
Tonlorenzi, Rossana;Dellavalle, Arianna;Sampaolesi, Maurilio
中血管母细胞是新近发现的干/祖细胞，与哺乳动物中胚层的小血管相关。最初在小鼠胚胎背主动脉中描述的，相似但不完全相同的细胞后来从出生后骨骼肌和心脏的小血管中被鉴定和表征(本单元中没有描述)。它们具有共同的解剖位置、内皮和/或周细胞标志物的表达、在培养中增殖的能力以及在适当的培养条件下分化为各种类型的中胚层细胞的能力。目前，中血管母细胞的发育起源、表型异质性以及与其他中胚层干细胞的关系尚不清楚，是目前活跃研究的主题。然而，从实践的角度来看，这些细胞已经成功地应用于细胞移植方案，显著挽救了营养不良的小鼠和狗的骨骼肌的结构和功能。由于最近已经分离和鉴定了相应的人类细胞，因此计划在不久的将来对这些细胞进行临床试验。本单元提供了分离、培养和鉴定中血管母细胞的详细方法。
Mesoangioblasts are recently identified stem/progenitor cells, associated with small vessels of the mesoderm in mammals. Originally described in the mouse embryonic dorsal aorta, similar though not identical cells have been later identified and characterized from postnatal small vessels of skeletal muscle and heart (not described in this unit). They have in common the anatomical location, the expression of endothelial and/or pericyte markers, the ability to proliferate in culture, and the ability to undergo differentiation into various types of mesoderm cells upon proper culture conditions. Currently, the developmental origin of mesoangioblasts, their phenotypic heterogeneity, and the relationship with other mesoderm stem cells are not understood in detail and are the subject of active research. However, from a practical point of view, these cells have been successfully used in cell transplantation protocols that have yielded a significant rescue of structure and function in skeletal muscle of dystrophic mice and dogs. Since the corresponding human cells have been recently isolated and characterized, a clinical trial with these cells is planned in the near future. This unit provides detailed methods for isolation, culture, and characterization of mesoangioblasts.