Native electrospray mass spectrometry of DNA G-quadruplexes in potassium solution.
Native electrospray mass spectrometry of DNA G-quadruplexes in potassium solution.
复制标题
钾溶液中 DNA G 四链体的非变性电喷雾质谱分析。
DOI：
10.1007/s13361-014-0890-3
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发表时间：
2014-07
影响因子：
3.2
通讯作者：
Gabelica, Valerie
中科院分区：
文献类型：
--
作者：
Marchand, Adrien;Gabelica, Valerie
关键词：
A commonly used electrolyte in electrospray mass spectrometry (ESI-MS) of biomolecules is ammonium acetate (NH4OAc). Although some nucleic acid structures such as duplexes require only proper physiological ionic strength (whatever the monovalent ions) to be properly folded in ESI-MS conditions, the folding of some other nucleic acid structures such as DNA G-quadruplexes also depends on direct binding of specific cations. Here, we developed ESI-MS compatible conditions that allow one to observe DNA G-quaduplexes with K+ ions specifically bound between G-quartets. NH4OAc was replaced with trimethylammonium acetate (TMAA), at concentrations up to 150 mM to provide physiological ionic strength, and the solution was doped with KCl at concentrations up to 1 mM. The trimethylammonium ion is too large to coordinate between G-quartets, where only K+ ions bind. Compared with the equivalent NH4OAc/KCl mixtures, the TMAA/KCl mixtures provide cleaner spectra by suppressing the nonspecific adducts, and favor the formation of similar stacking arrangements as in 100 mM KCl (physiologically relevant cation) for the polymorphic human telomeric DNA G-quadruplexes. This new sample preparation method can be exploited to determine the number of potassium binding sites in new sequences, to screen ligand binding to the structures favored in potassium, and to transfer potassium-bound G-quadruplexes to the mass spectrometer for gas-phase structural probing, as illustrated herein with ion mobility spectrometry experiments. ᅟ The online version of this article (doi:10.1007/s13361-014-0890-3) contains supplementary material, which is available to authorized users.
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影响因子：
14.9
作者：
Cavaluzzi, MJ;Borer, PN
通讯作者：
Borer, PN
影响因子：
14.9
作者：
POTIER, N;VANDORSSELAER, A;BISCHOFF, R
通讯作者：
BISCHOFF, R
影响因子：
14.9
作者：
Ambrus A;Chen D;Dai J;Bialis T;Jones RA;Yang D
通讯作者：
Yang D
影响因子：
4.3
作者：
Pierce, Sarah E.;Wang, Junmei;Jayawickramarajah, Janarthanan;Hamilton, Andrew D.;Brodbelt, Jennifer S.
通讯作者：
Brodbelt, Jennifer S.
影响因子：
14.9
作者：
Gros J;Rosu F;Amrane S;De Cian A;Gabelica V;Lacroix L;Mergny JL
通讯作者：
Mergny JL