Interaction of calcium and neomycin with anionic phospholipid-lecithin liposomes. A differential scanning calorimetry study.
Interaction of calcium and neomycin with anionic phospholipid-lecithin liposomes. A differential scanning calorimetry study.
复制标题
钙和新霉素与阴离子磷脂-卵磷脂脂质体的相互作用。
DOI：
10.1016/0006-2952(84)90041-8
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发表时间：
1984
影响因子：
5.8
通讯作者：
Schacht,J
中科院分区：
文献类型：
--
作者：
Wang,BM;Weiner,ND;Ganesan,MG;Schacht,J
用差示扫描量热法研究了钙和新霉素与不同阴离子磷脂+卵磷脂脂质体的相互作用。磷脂酰肌醇二磷酸与钙和新霉素的相互作用不同于其他酸性磷脂。钙在低至1 mM的浓度下，仅在磷脂酰肌醇二磷酸脂质体中诱导出现第二过渡峰。新霉素起拮抗作用，阻止了这种相分离。此外，新霉素降低了磷脂酰肌醇二磷酸-卵磷脂脂质体的相变温度，而提高了所有受试阴离子磷脂-卵磷脂脂质体的相变温度。新霉素的这种流态化作用和对钙离子的拮抗作用可能会引起生物膜性质的严重变化。这项研究支持并扩展了我们之前的发现和结论，即磷脂酰肌醇二磷酸可能在氨基糖苷类毒性的表达中发挥关键作用。
The interactions of calcium and neomycin with liposomes of various anionic phospholipids plus lecithin were studied by differential scanning calorimetry. Phosphatidylinositol bisphosphate differed from other acidic phospholipids in its interactions with both calcium and neomycin. Calcium, at concentrations as low as 1 mM, induced the appearance of a second transition peak in phosphatidylinositol bisphosphate-enriched liposomes only. Neomycin acted antagonistically and precluded this phase separation. In addition, neomycin lowered the phase transition temperature of phosphatidylinositol bisphosphate-lecithin liposomes while it raised the transition temperature of all other anionic phospholipid-lecithin liposomes tested. This fluidizing effect of neomycin and the antagonism to calcium may induce critical alterations of properties of biological membranes. The study supports and extends our previous findings and conclusions that phosphatidylinositol bisphosphate may play a crucial role in the expression of aminoglycoside toxicity.