Genetic study of human cells in vitro. Carbohydrate variants from cultures of HeLa and conjunctival cells.
Genetic study of human cells in vitro. Carbohydrate variants from cultures of HeLa and conjunctival cells.
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DOI：
10.1084/jem.111.2.235
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发表时间：
1960-02-01
期刊：
影响因子：
--
通讯作者：
CHANG RS
中科院分区：
文献类型：
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作者：
CHANG RS
描述了从HeLa和结膜细胞培养物中分离碳水化合物变体。细胞培养系统中固有的因素，如亲本群体和透析血清，已被证明会影响变异体分离的结果。建立的稳定的变体掺入显着更多的戊糖或乳酸到各种细胞级分比亲本培养物。除了它们在选择环境中连续繁殖的能力之外，变异体繁殖较慢，更容易受到零度以下保存和D-2-脱氧葡萄糖的细胞毒性作用的影响，表现出较低的克隆效率，并且对葡萄糖氧化酶的有害作用不太敏感。核糖变体在形态学外观上也不同于亲本培养物，例如形成多核细胞和环形集落。它们比亲本培养物将更多的核糖转化为粘多糖的其他组分糖。从核糖变体和亲本培养物中提取的粘多糖的初步分析显示其碳水化合物（Molisch阳性材料）和DNA比率存在很大差异。有证据表明，从遗传选择到原始形态发生的一系列相互关联的事件已经确立。
The isolation of carbohydrate variants from cultures of HeLa and conjunctival cells was described. Factors inherent in the cell culture system, such as parent populations and dialyzed serums, have been shown to influence the outcome of variant isolations. Established stable variants incorporated significantly more pentoses or lactate into various cell fractions than the parent cultures. Besides their abilities to propagate continuously in the selecting environments, the variants multiplied slower, were more susceptible to sub-zero preservation and the cytotoxic effect of D-2-deoxyglucose, showed lower cloning efficiencies and were less susceptible to the deleterious effect of glucose oxidase. The ribose variants also differed from the parent cultures in morphological appearance such as formation of multinucleated cells and ring-shaped colonies. They converted more ribose into other component sugars of mucopolysaccharides than the parent cultures. Preliminary analyses of the mucopolysaccharides extracted from the ribose variants and parent cultures showed large difference in their carbohydrate (Molisch-positive materials) and DNA ratios. Evidence suggests that a sequence of interrelated events from genetic selection to primitive morphogenesis has been established.