An efficient method for the isolation of intramuscular collagen.
An efficient method for the isolation of intramuscular collagen.
复制标题
分离肌内胶原蛋白的有效方法。
DOI：
10.1016/0309-1740(94)00067-h
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发表时间：
1995
期刊：
影响因子：
7.1
通讯作者：
A. Bailey
中科院分区：
文献类型：
--
作者：
N. Avery;A. Bailey
肌原纤维的超声破碎和随后在缓冲液中的溶解已被用于以高产率和纯度分离肌内胶原（IMC）。该方法在提供高产量的胶原蛋白和完整的纤维方面上级先前报道的技术。所获得的材料适用于物理和生物化学分析，试图证明其在确定肉的嫩度中的作用。
Ultrasonic fragmentation of the myofibrils and subsequent solubilisation in buffer has been used to isolate intramuscular collagen (IMC) in high yield and purity. The method is superior to previously reported techniques in providing both a high yield of collagen and intact fibres. The material obtained is suitable for both physical and biochemical analysis in attempts to demonstrate its role in determining the tenderness of meat.