The Brassicaceae-specific secreted peptides, STMPs, function in plant growth and pathogen defense
The Brassicaceae-specific secreted peptides, STMPs, function in plant growth and pathogen defense
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十字花科特异性分泌肽 STMP 在植物生长和病原体防御中发挥作用
DOI：
10.1111/jipb.12817
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发表时间：
2019
影响因子：
11.4
通讯作者：
Zheng Chengchao
中科院分区：
文献类型：
--
作者：
Yu Zipeng;Xu Yang;Zhu Lifei;Zhang Lei;Liu Lin;Zhang Di;Li D;an;Wu Changai;Huang Jinguang;Yang Guodong;Yan Kang;Zhang Shizhong;Zheng Chengchao
低分子量分泌肽最近已被证明影响植物生长、发育和防御反应的多个方面。在这里，我们进行了逐步BLAST过滤，以确定未注释的肽从拟南芥蛋白质数据库，并发现了一个新的分泌肽家族，分泌跨膜肽（STMPs）。这些低分子量肽由N末端信号肽和跨膜结构域组成，主要定位于细胞外区室，但也在分泌途径的内膜系统（包括内质网和高尔基体）中检测到。综合生物信息学分析确定了10个STMP家族成员，是特定的菊科。化学合成的STMP1和STMP2显著抑制菊科植物根系的生长，表达STMP1、2、4、6和10的拟南芥根系生长受到严重抑制，表明STMPs参与了植物生长发育的调控。此外，体外生物活性测定表明，STMP1、STMP2和STMP10对番茄假单胞菌DC3000、青枯菌、枯草芽孢杆菌和根癌农杆菌具有抗菌作用，表明STMPs是一种抗菌肽。这些结果表明，STMP家族成员在菊科植物的各种发育事件和病原防御反应中发挥重要作用。
Low molecular weight secreted peptides have recently been shown to affect multiple aspects of plant growth, development, and defense responses. Here, we performed stepwise BLAST filtering to identify unannotated peptides from theArabidopsis thalianaprotein database and uncovered a novel secreted peptide family, secreted transmembrane peptides (STMPs). These low molecular weight peptides, which consist of an N‐terminal signal peptide and a transmembrane domain, were primarily localized to extracellular compartments but were also detected in the endomembrane system of the secretory pathway, including the endoplasmic reticulum and Golgi. Comprehensive bioinformatics analysis identified 10 STMP family members that are specific to the Brassicaceae family. Brassicaceae plants showed dramatically inhibited root growth upon exposure to chemically synthesized STMP1 and STMP2.ArabidopsisoverexpressingSTMP1,2,4,6, or10exhibited severely arrested growth, suggesting that STMPs are involved in regulating plant growth and development. In addition,in vitrobioassays demonstrated that STMP1, STMP2, and STMP10 have antibacterial effects againstPseudomonas syringaepv.tomatoDC3000,Ralstonia solanacearum,Bacillus subtilis, andAgrobacterium tumefaciens, demonstrating that STMPs are antimicrobial peptides. These findings suggest that STMP family members play important roles in various developmental events and pathogen defense responses in Brassicaceae plants.