Polygenic powdery mildew disease resistance in Arabidopsis thaliana:: quantitative trait analysis of the accession Warschau-1
Polygenic powdery mildew disease resistance in Arabidopsis thaliana:: quantitative trait analysis of the accession Warschau-1
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DOI：
10.1046/j.1365-3059.2001.00611.x
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发表时间：
2001-12-01
期刊：
影响因子：
2.7
通讯作者：
Somerville, SC
中科院分区：
文献类型：
--
作者：
Schiff, CL;Wilson, IW;Somerville, SC
为了进一步了解拟南芥抗白粉病（Erysiphe cichoracearum）的天然遗传多样性，对来自抗性登录Warschau-1和感病哥伦比亚-0之间杂交的重组自交系进行了数量性状位点分析。白粉病在Warschau-1上生长不太好，但抗性与感染序列中的特定块无关。两个潜在的白粉病抗病基因座进行了鉴定和定位，一个主要的影响，一个轻微的影响抗病性。这两个位点以加性方式赋予抗性，它们共同解释了65%的抗性变异。此外，主要的白粉病抗病基因座遗传定位到染色体III的底部，一个区域包含白粉病抗性基因座RPW 7，RPW 8和RPW 10。与登记莫斯科-1中RPW 8位点介导的抗性不同，Warschau-1中的抗性与过敏反应无关，突出了遗传背景或环境因素对抗病性表达的影响。结合其他研究中所描述的白粉病抗性位点，这些结果表明A.拟南芥是一种有用的天然抗白粉病资源，其潜在地可用于基础研究和作为应用研究的工具。
To further the understanding of the natural genetic diversity for disease resistance to powdery mildew (Erysiphe cichoracearum) in Arabidopsis thaliana, quantitative trait loci analysis was undertaken on recombinant inbred lines derived from a cross between the resistant accession Warschau-1 and the susceptible Columbia-0. Powdery mildew grew less well on Warschau-1, but the resistance was not associated with a specific block in the infection sequence. Two potential powdery mildew disease-resistance loci were identified and mapped, one with a major effect and one with a minor effect on disease resistance. The two loci acted in an additive manner to confer resistance, and together they explained 65% of the variation in resistance. In addition, the major powdery mildew disease-resistance locus was genetically mapped to the bottom of chromosome III, a region containing the powdery mildew resistance loci RPW7, RPW8 and RPW10. Unlike resistance mediated by the RPW8 locus in the accession Moscow-1, resistance in Warschau-1 was not correlated with the hypersensitive response, highlighting the influence of genetic background or environmental factors on the expression of disease resistance. Together with the powdery mildew resistance loci described in other studies, these results suggest that A. thaliana is a useful source of natural powdery mildew disease resistance, which potentially can be utilized in fundamental studies and as a tool for applied studies.