Food preservative sorbic acid deregulates hepatic fatty acid metabolism.
Food preservative sorbic acid deregulates hepatic fatty acid metabolism.
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DOI：
10.38212/2224-6614.1055
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发表时间：
2020-06-15
影响因子：
3.6
通讯作者：
Lee, Tzong-Shyuan
中科院分区：
文献类型：
--
作者：
Chen, Chia-Hui;Ho, Sin-Ni;Hu, Po-An;Kou, Yu Ru;Lee, Tzong-Shyuan
Sorbic acid (SA) is one of the most commonly used food preservatives worldwide. Despite SA having no hepatotoxicity at legal dosages, its effect on hepatic lipid metabolism is still unclear. We investigated the effect of SA on hepatic lipid metabolism and its mechanism of action in C57BL/6 mice. Daily treatment with SA (1 g/kg in diet) for 4 weeks did not alter the body weight, organ weight, and blood lipids in mice. However, hepatic lipid accumulation, particularly that of triglycerides, fatty acids, and glycerol, but not cholesteryl ester and free cholesterol, was increased with SA treatment. Mechanistically, SA decreased the expression of proteins related to de novo fatty acid lipogenesis, fatty acid internalization, and very low-density lipoprotein (VLDL) secretion-related pathways, including sterol regulatory element-binding proteins, acetyl-coA carboxylase, fatty acid synthase, liver fatty acid-binding protein, CD36, and apolipoprotein E. In contrast, SA increased the expression of diacylglycerol O-acyltransferase 2, the key enzyme for triacylglycerol synthesis. Moreover, SA downregulated the protein expression of autophagy-related and β-oxidation-related pathways, the two major metabolic pathways for lipid metabolism, including LC-3, beclin-1, autophagy related protein 5 (ATG-5) and ATG-7, acyl-CoA synthetase long chain family member 1, carnitine palmitoyltransferase Iα, peroxisome proliferator-activated receptor α (PPARα), PPARγ, and PPARγ coactivator-1. Collectively, SA deregulates de novo lipogenesis and fatty acid internalization, VLDL secretion, autophagy, and β-oxidation in the liver, leading to impaired lipid clearance and ultimately, resulting in lipid accumulation in the liver.
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影响因子：
6.5
作者：
Irshad Z;Chmel N;Adya R;Zammit VA
通讯作者：
Zammit VA
影响因子：
6.1
作者：
Ling, Min-Pei;Lien, Keng-Wen;Hsieh, Dennis P. H.
通讯作者：
Hsieh, Dennis P. H.
影响因子：
3.2
作者：
Kawahata, Miho;Masaki, Kazuo;Iefuji, Haruyuki
通讯作者：
Iefuji, Haruyuki
DOI：
10.1007/s00018-018-2860-6
发表时间：
2018-09
期刊：
Cellular and molecular life sciences : CMLS
影响因子：
--
作者：
Ipsen DH;Lykkesfeldt J;Tveden-Nyborg P
通讯作者：
Tveden-Nyborg P
影响因子：
8.9
作者：
Koo SH
通讯作者：
Koo SH