EFFECTS OF ISRADIPINE ON ENDOTHELIN-INDUCED CONSTRICTION OF MYOMETRIAL ARTERIES IN NORMOTENSIVE PREGNANT-WOMEN
EFFECTS OF ISRADIPINE ON ENDOTHELIN-INDUCED CONSTRICTION OF MYOMETRIAL ARTERIES IN NORMOTENSIVE PREGNANT-WOMEN
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DOI：
10.1093/ajh/7.7.50s
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发表时间：
1994-07-01
影响因子：
3.2
通讯作者：
NISELL, H
中科院分区：
文献类型：
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作者：
KUBLICKIENE, KR;WOLFF, K;NISELL, H
我们之前的研究结果表明，内皮素-1 （ET-1）可能与子痫前期子宫胎盘血流受损有关。本研究的目的是研究isradipine对et -1诱导的孕妇子宫肌阻力动脉收缩的体外影响，因为这些血管在子痫前期部分负责子宫胎盘血流的调节。从20名接受择期剖宫产术的血压正常的足月孕妇的子宫肌层上剥离小的子宫肌动脉，并将其安装在组织室中。张力被等距记录。当ET-1 （10-(8) mol/L）收缩的血管暴露于增加浓度（10（-6）、10（-5）和10(-4)mol/L）的isradipine时，肌内膜动脉基本没有松弛。只有当isradipine浓度达到10(-3)mol/L时，才有31%的弛豫。isradipine预处理在3 × 10(-4) mol/L时可减弱26%的et -1诱导的收缩，在10(-3)mol/L时可减弱80%。较低浓度的isradipine未显著降低ET-1的收缩。因此，目前的研究表明，高浓度的isradipine可以抵消足月孕妇et -1诱导的肌内膜动脉收缩。高浓度isradipine预处理可减弱ET-1收缩。
The results of our previous studies suggested that endothelin-1 (ET-1) might be contributory to the impaired uteroplacental blood flow seen in preeclampsia. The aim of this study was to investigate the in vitro influence of isradipine on ET-1-induced contraction of myometrial resistance arteries from pregnant women, as these vessels are partly responsible for the regulation of uteroplacental blood flow in preeclampsia.Small myometrial arteries were dissected from myometrium obtained from 20 normotensive term pregnant women undergoing elective cesarean section and mounted in a tissue chamber. Tension was recorded isometrically. When ET-1 (10-(8) mol/L)-contracted vessels were exposed to increasing concentrations (10(-6), 10(-5), and 10(-4) mol/L) of isradipine, the myometrial arteries demonstrated essentially no relaxation. A significant mean relaxation of 31% was seen only with the highest isradipine concentration of 10(-3) mol/L. Pretreatment with isradipine attenuated ET-1-induced contraction by 26% at 3 x 10(-4) mol/L and by up to 80% at 10(-3) mol/L. Preincubation with lower concentrations of isradipine did not significantly reduce subsequent ET-1 contraction.The present study has thus shown that isradipine at high concentrations counteracts ET-1-induced constriction of myometrial arteries in term pregnant women. Pretreatment with isradipine at high concentrations attenuates the ET-1 contraction.