Microbial community dynamics during start-up operation of flowerpot-using fed-batch reactors for composting of household biowaste
Microbial community dynamics during start-up operation of flowerpot-using fed-batch reactors for composting of household biowaste
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DOI：
10.1046/j.1462-2920.2003.00473.x
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发表时间：
2003-09-01
影响因子：
5.1
通讯作者：
Yamanaka, Y
中科院分区：
文献类型：
--
作者：
Hiraishi, A;Narihiro, T;Yamanaka, Y
采用醌类化合物分析法、rRNA荧光原位杂交（FISH）技术和培养方法，研究了生活垃圾堆肥花盆式流加反应器启动过程中微生物群落的变化。在启动过程中，反应器中细菌总数、菌落数和醌含量均随时间急剧增加。这些增加模式有两个阶段;第一次增加发生在从废物装载开始的3-4周期间，第二次增加发生在随后的4周期间。微生物生物量在两个演替阶段之间暂时减少。泛醌占主导地位，在开始运作，但随着时间的推移急剧下降，而部分饱和的甲基萘醌成为占主导地位的完全驯化阶段。这些数据表明，在运行期间，微生物种群的主要组成从含泛醌的变形菌转变为放线菌。邻加入树状图构建的醌配置文件数据的基础上建议，至少需要一个月的时间来建立一个稳定的社区结构与放线菌占主导地位。启动过程中的特征性群体转移也通过FISH探测和分离的细菌菌株的16 S rDNA序列比较来证明。
Microbial community changes during start-up operation of flowerpot-using fed-batch reactors for composting of household biowaste were studied by quinone profiling, rRNA-targeted fluorescence in situ hybridization (FISH) and cultivation methods. Total and plate counts of bacteria and quinone contents in the reactors increased sharply with time during the start-up period. These increase patterns had two phases; the first increase occurred during 3-4 weeks from the start of waste loading and the second increase was found during the subsequent 4 weeks. The microbial biomass was temporally reduced between the two succession phases. Ubiquinones predominated at the beginning of operation but decreased sharply with time, whereas partially saturated menaquinones became predominant at the fully acclimated stage. These data indicated that the major constituents of microbial populations changed from ubiquinone-containing Proteobacteria to Actinobacteria during the period of operation. Neighbour-joining dendrograms constructed based on the quinone profile data suggested that at least one month is required to establish a stable community structure with the Actinobacteria predominating. The characteristic population shift in the start-up process was also demonstrated by FISH probing and 16S rDNA sequence comparisons of bacterial strains isolated.