Simultaneous assessment of rodent behavior and neurochemistry using a miniature positron emission tomograph
Simultaneous assessment of rodent behavior and neurochemistry using a miniature positron emission tomograph
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DOI：
10.1038/nmeth.1582
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发表时间：
2011-04-01
期刊：
影响因子：
48
通讯作者：
Vaska, Paul
中科院分区：
文献类型：
--
作者：
Schulz, Daniela;Southekal, Sudeepti;Vaska, Paul
啮齿类动物的正电子发射断层扫描 (PET) 神经成像和行为测定广泛应用于神经科学。 PET 可以深入了解神经元通讯的分子过程，而行为方法可以分析与此类过程相关的行为。这些方法尚未直接整合，因为迄今为止，动物 PET 研究需要全身麻醉来固定受试者，这妨碍了行为研究。我们提出了一种用 PET 对清醒、有行为的大鼠进行成像的方法，可以同时研究行为。关键组件包括“大鼠意识动物 PET”或 RatCAP，这是一种安装在大鼠头部的微型便携式 PET 扫描仪、一个允许相当大的活动自由的移动系统、放射性示踪剂管理技术以及用于量化行为和关联两个数据集的方法。 PET 和行为数据的同时性为研究不同大脑区域的功能及其分子成分提供了多维工具。
Positron emission tomography (PET) neuroimaging and behavioral assays in rodents are widely used in neuroscience. PET gives insights into the molecular processes of neuronal communication, and behavioral methods analyze the actions that are associated with such processes. These methods have not been directly integrated, because PET studies in animals have until now required general anesthesia to immobilize the subject, which precludes behavioral studies. We present a method for imaging awake, behaving rats with PET that allows the simultaneous study of behavior. Key components include the 'rat conscious animal PET' or RatCAP, a miniature portable PET scanner that is mounted on the rat's head, a mobility system that allows considerable freedom of movement, radiotracer administration techniques and methods for quantifying behavior and correlating the two data sets. The simultaneity of the PET and behavioral data provides a multidimensional tool for studying the functions of different brain regions and their molecular constituents.