miR-122 direct detection in human serum by time-gated fluorescence imaging
miR-122 direct detection in human serum by time-gated fluorescence imaging
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DOI：
10.1039/c9cc08069d
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发表时间：
2019-12-25
影响因子：
4.9
通讯作者：
Orte, Angel
中科院分区：
文献类型：
--
作者：
Garcia-Fernandez, Emilio;Carmen Gonzalez-Garcia, M.;Orte, Angel
提出了一种不使用聚合酶扩增直接检测人血清中microRNAs （miRs）的简单方法，实现了低miR-122浓度，重要的是，有效识别单碱基序列突变。该方法基于合成肽核酸低聚物捕获目标miRs，动态化学标记，季胺微平台分离和时间门控荧光成像检测。
A simple method for direct detection of microRNAs (miRs) in human serum without the use of polymerase amplification is presented, achieving low miR-122 concentrations and importantly, discerning effectively single-base sequence mutations. The method is based on the capture of target miRs with synthetic peptide nucleic acid oligomers, dynamic chemical labelling, separation with quaternary amine microplatforms and detection using time-gated fluorescence imaging.