MAP4K3 regulates body size and metabolism in Drosophila
MAP4K3 regulates body size and metabolism in Drosophila
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DOI：
10.1016/j.ydbio.2010.04.027
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发表时间：
2010-08-01
影响因子：
2.7
通讯作者：
Teleman, Aurelio A.
中科院分区：
文献类型：
--
作者：
Bryk, Boris;Hahn, Katrin;Teleman, Aurelio A.
TOR途径介导动物细胞生长和代谢的营养响应调节，TOR复合体1的活性依赖于氨基酸的可获得性，其中MAP4K3最近参与了细胞培养中的氨基酸信号转导。本文报道了缺乏MAP4K3的突变果蝇的生理特征，通过S6K和4EBP的磷酸化检测到TORC1活性降低。此外，MAP4K3突变体表现出低TORC1活性和低养分利用率的表型特征，如生长速度减慢，体型较小，以及低脂储备当动物在低营养条件下饲养时，对照和MAP4K3突变动物之间的差异缩小。最后，我们展示了MAP4K3和RAG GTP酶之间的物理相互作用，提高了它们可能作用于一个信号通路的可能性(C)2010 Elsevier Inc.保留所有权利
The TOR pathway mediates nutrient-responsive regulation of cell growth and metabolism in animals TOR Complex 1 activity depends, amongst other things, on amino acid availability MAP4K3 was recently implicated in amino-acid signaling in cell culture We report here the physiological characterization of MAP4K3 mutant flies Flies lacking MAP4K3 have reduced TORC1 activity detected by phosphorylation of S6K and 4EBP Furthermore MAP4K3 mutants display phenotypes characteristic of low TORC1 activity and low nutrient availability, such as reduced growth rate, small body size, and low lipid reserves The differences between control and MAP4K3 mutant animals diminish when animals are reared in low-nutrient conditions. suggesting that the ability of TOR to sense amino acids is most important when nutrients are abundant Lastly, we show physical interaction between MAP4K3 and the Rag GTPases raising the possibility they might be acting in one signaling pathway (C) 2010 Elsevier Inc All rights reserved