The dentinogenic effect of mineral trioxide aggregate (MTA) in short-term capping experiments
The dentinogenic effect of mineral trioxide aggregate (MTA) in short-term capping experiments
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DOI：
10.1046/j.1365-2591.2002.00471.x
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发表时间：
2002-03-01
影响因子：
5
通讯作者：
Papadimitriou, S
中科院分区：
文献类型：
--
作者：
Tziafas, D;Pantelidou, O;Papadimitriou, S
目的研究机械暴露牙髓盖敷MTA后早期牙髓细胞反应及修复性牙本质形成的发生。方法选取3只12-18月龄犬33颗牙，经V类牙洞机械暴露。轻压控制出血。prooroot MTA （Dentsply Simfra, Paris）放置在曝光部位，用湿棉球施加光压。采用银汞合金修复龋洞，在愈合间隔1、2、3周后，通过光镜和电子显微镜（透射和扫描）观察牙髓组织的反应。结果在髓质- mta界面上初步发现了一个均匀的晶体结构区，同时显示细胞学和功能状态变化的髓质细胞被排列在靠近晶体的位置。在与盖层材料和相关晶体结构直接接触的所有牙齿中均发现骨型硬组织沉积。修复性牙本质的形成（由细长极化细胞以极似牙本质的方式形成管状基质）始终与坚固的骨牙本质区有关。结论MTA是一种有效的牙髓盖盖材料，可通过牙髓创伤早期愈合的刻板防御机制刺激修复性牙本质的形成。
Aim The objective of the present experiment was to study the early pulpal cell response and the onset of reparative dentine formation after capping application of MTA in mechanically exposed pulps.Methodology Thirty-three teeth from three dogs, 12-18 months of age were mechanically exposed via class V cavities. Light pressure was applied to control haemorrhage. ProRoot MTA (Dentsply Simfra, Paris) was placed at the exposure site and light pressure was applied with a wet cotton pellet. The cavities were restored with amalgam and the pulpal tissue reactions were assessed by light and electron microscopy (transmission and scanning) after healing intervals of 1, 2 or 3 weeks.Results A homogenous zone of crystalline structures was initially found along the pulp-MTA interface, whilst pulpal cells showing changes in their cytological and functional state were arranged in close proximity to the crystals. Deposition of hard tissue of osteotypic form was found in all teeth in direct contact with the capping material and the associated crystalline structures. Formation of reparative dentine (tubular matrix formation in a polar predentine-like pattern by elongated polarized cells) was consistently related to a firm osteodentinal zone.Conclusions The present experiments indicate that MTA is an effective pulp-capping material, able to stimulate reparative dentine formation by the stereotypic defensive mechanism of early pulpal wound healing.