Cytosolic fumarase acts as a metabolic fail-safe for both high and low temperature acclimation of Arabidopsis thaliana
Cytosolic fumarase acts as a metabolic fail-safe for both high and low temperature acclimation of Arabidopsis thaliana
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DOI：
10.1101/2021.04.19.440416
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发表时间：
2021-04
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影响因子：
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通讯作者：
Helena A. Herrmann;P. Calzadilla;J. Schwartz;G. Johnson
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文献类型：
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作者：
Helena A. Herrmann;P. Calzadilla;J. Schwartz;G. Johnson
植物适应不断变化的环境条件下的光合作用能力。在拟南芥中，适应低温的光合作用需要富马酸的积累，而富马酸是由胞质富马酸酶FUM2催化的。然而，这种积累的作用目前尚不清楚。在这项研究中，我们使用一种综合的实验和建模方法来研究FUM2和富马酸在生理温度范围内的作用。通过生理生化分析，我们证明了FUM2对于高温驯化和低温驯化都是必要的。我们采用了一种可靠性工程技术，故障模式和影响分析(FMEA)，以形成一种严格的方法，根据代谢物对代谢系统构成的潜在风险对其进行排序。FMEA确定富马酸盐为低风险代谢物，而其前体苹果酸被证明是高风险的，容易导致系统不稳定。我们的结论是，胞质富马酸酶FUM2的作用是提供故障保护，控制苹果酸浓度，在不断变化的环境中保持系统的稳定性。我们认为，FMEA是一种不仅对了解植物新陈代谢有用的技术，而且还可以用于研究其他系统和合成途径的可靠性。
Plants acclimate their photosynthetic capacity in response to changing environmental conditions. In Arabidopsis thaliana, photosynthetic acclimation to cold requires the accumulation of the organic acid fumarate, catalysed by a cytosolic fumarase FUM2. However, the role of this accumulation is currently unknown. In this study, we use an integrated experimental and modelling approach to examine the role of FUM2 and fumarate across the physiological temperature range. Using physiological and biochemical analyses, we demonstrate that FUM2 is necessary for high as well as low temperature acclimation. We have adapted a reliability engineering technique, Failure Mode and Effect Analysis (FMEA), to formalize a rigorous approach for ranking metabolites according to the potential risk that they pose to the metabolic system. FMEA identifies fumarate as a low-risk metabolite, while its precursor, malate, is shown to be high-risk and liable to cause system instability. We conclude that the role of cytosolic fumarase, FUM2, is to provide a fail-safe, controlling malate concentration, maintaining system stability in a changing environment. We argue that FMEA is a technique that is not only useful in understanding plant metabolism but can also be used to study reliability in other systems and synthetic pathways.